1rxm
From Proteopedia
(New page: 200px<br /><applet load="1rxm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rxm, resolution 2.8Å" /> '''C-terminal region of ...) |
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| - | [[Image:1rxm.gif|left|200px]]<br /><applet load="1rxm" size=" | + | [[Image:1rxm.gif|left|200px]]<br /><applet load="1rxm" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1rxm, resolution 2.8Å" /> | caption="1rxm, resolution 2.8Å" /> | ||
'''C-terminal region of FEN-1 bound to A. fulgidus PCNA'''<br /> | '''C-terminal region of FEN-1 bound to A. fulgidus PCNA'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Flap EndoNuclease-1 (FEN-1) and the processivity factor proliferating cell | + | Flap EndoNuclease-1 (FEN-1) and the processivity factor proliferating cell nuclear antigen (PCNA) are central to DNA replication and repair. To clarify the molecular basis of FEN-1 specificity and PCNA activation, we report here structures of FEN-1:DNA and PCNA:FEN-1-peptide complexes, along with fluorescence resonance energy transfer (FRET) and mutational results. FEN-1 binds the unpaired 3' DNA end (3' flap), opens and kinks the DNA, and promotes conformational closing of a flexible helical clamp to facilitate 5' cleavage specificity. Ordering of unstructured C-terminal regions in FEN-1 and PCNA creates an intermolecular beta sheet interface that directly links adjacent PCNA and DNA binding regions of FEN-1 and suggests how PCNA stimulates FEN-1 activity. The DNA and protein conformational changes, composite complex structures, FRET, and mutational results support enzyme-PCNA alignments and a kinked DNA pivot point that appear suitable to coordinate rotary handoffs of kinked DNA intermediates among enzymes localized by the three PCNA binding sites. |
==About this Structure== | ==About this Structure== | ||
| - | 1RXM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http:// | + | 1RXM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RXM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Archaeoglobus fulgidus]] | [[Category: Archaeoglobus fulgidus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chapados, B | + | [[Category: Chapados, B R.]] |
[[Category: Han, S.]] | [[Category: Han, S.]] | ||
| - | [[Category: Hosfield, D | + | [[Category: Hosfield, D J.]] |
[[Category: Qiu, J.]] | [[Category: Qiu, J.]] | ||
[[Category: Shen, B.]] | [[Category: Shen, B.]] | ||
| - | [[Category: Tainer, J | + | [[Category: Tainer, J A.]] |
[[Category: Yelent, B.]] | [[Category: Yelent, B.]] | ||
[[Category: beta-zipper]] | [[Category: beta-zipper]] | ||
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[[Category: torus]] | [[Category: torus]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:55:37 2008'' |
Revision as of 12:55, 21 February 2008
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C-terminal region of FEN-1 bound to A. fulgidus PCNA
Overview
Flap EndoNuclease-1 (FEN-1) and the processivity factor proliferating cell nuclear antigen (PCNA) are central to DNA replication and repair. To clarify the molecular basis of FEN-1 specificity and PCNA activation, we report here structures of FEN-1:DNA and PCNA:FEN-1-peptide complexes, along with fluorescence resonance energy transfer (FRET) and mutational results. FEN-1 binds the unpaired 3' DNA end (3' flap), opens and kinks the DNA, and promotes conformational closing of a flexible helical clamp to facilitate 5' cleavage specificity. Ordering of unstructured C-terminal regions in FEN-1 and PCNA creates an intermolecular beta sheet interface that directly links adjacent PCNA and DNA binding regions of FEN-1 and suggests how PCNA stimulates FEN-1 activity. The DNA and protein conformational changes, composite complex structures, FRET, and mutational results support enzyme-PCNA alignments and a kinked DNA pivot point that appear suitable to coordinate rotary handoffs of kinked DNA intermediates among enzymes localized by the three PCNA binding sites.
About this Structure
1RXM is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
Reference
Structural basis for FEN-1 substrate specificity and PCNA-mediated activation in DNA replication and repair., Chapados BR, Hosfield DJ, Han S, Qiu J, Yelent B, Shen B, Tainer JA, Cell. 2004 Jan 9;116(1):39-50. PMID:14718165
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