1ryj
From Proteopedia
(New page: 200px<br /><applet load="1ryj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ryj" /> '''Solution NMR Structure of Protein Mth1743 fr...) |
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| - | [[Image:1ryj.gif|left|200px]]<br /><applet load="1ryj" size=" | + | [[Image:1ryj.gif|left|200px]]<br /><applet load="1ryj" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''Solution NMR Structure of Protein Mth1743 from Methanobacterium thermoautotrophicum. Ontario Centre for Structural Proteomics target MTH1743_1_70; Northeast Structural Genomics Consortium Target TT526.'''<br /> | '''Solution NMR Structure of Protein Mth1743 from Methanobacterium thermoautotrophicum. Ontario Centre for Structural Proteomics target MTH1743_1_70; Northeast Structural Genomics Consortium Target TT526.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The influx of genomic sequence information has led to the concept of | + | The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1RYJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermococcus_thermolithotrophicus Methanothermococcus thermolithotrophicus]. Full crystallographic information is available from [http:// | + | 1RYJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanothermococcus_thermolithotrophicus Methanothermococcus thermolithotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RYJ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Methanothermococcus thermolithotrophicus]] | [[Category: Methanothermococcus thermolithotrophicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Arrowsmith, C | + | [[Category: Arrowsmith, C H.]] |
[[Category: Bhattacharyya, S.]] | [[Category: Bhattacharyya, S.]] | ||
[[Category: Chang, X.]] | [[Category: Chang, X.]] | ||
[[Category: Chen, L.]] | [[Category: Chen, L.]] | ||
| - | [[Category: Cort, J | + | [[Category: Cort, J R.]] |
[[Category: Denisov, A.]] | [[Category: Denisov, A.]] | ||
| - | [[Category: Edwards, A | + | [[Category: Edwards, A M.]] |
[[Category: Finak, G.]] | [[Category: Finak, G.]] | ||
[[Category: Gehring, K.]] | [[Category: Gehring, K.]] | ||
[[Category: Gutierrez, P.]] | [[Category: Gutierrez, P.]] | ||
| - | [[Category: Kennedy, M | + | [[Category: Kennedy, M A.]] |
[[Category: Kozlov, G.]] | [[Category: Kozlov, G.]] | ||
[[Category: Le, B.]] | [[Category: Le, B.]] | ||
| - | [[Category: Lee, C | + | [[Category: Lee, C H.]] |
| - | [[Category: Lee, G | + | [[Category: Lee, G M.]] |
[[Category: Lee, W.]] | [[Category: Lee, W.]] | ||
[[Category: Liao, J.]] | [[Category: Liao, J.]] | ||
| - | [[Category: McIntosh, L | + | [[Category: McIntosh, L P.]] |
| - | [[Category: NESG, Northeast | + | [[Category: NESG, Northeast Structural Genomics Consortium.]] |
[[Category: Pineda-Lucena, A.]] | [[Category: Pineda-Lucena, A.]] | ||
[[Category: Ramelot, T.]] | [[Category: Ramelot, T.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:55:55 2008'' |
Revision as of 12:55, 21 February 2008
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Solution NMR Structure of Protein Mth1743 from Methanobacterium thermoautotrophicum. Ontario Centre for Structural Proteomics target MTH1743_1_70; Northeast Structural Genomics Consortium Target TT526.
Overview
The influx of genomic sequence information has led to the concept of structural proteomics, the determination of protein structures on a genome-wide scale. Here we describe an approach to structural proteomics of small proteins using NMR spectroscopy. Over 500 small proteins from several organisms were cloned, expressed, purified, and evaluated by NMR. Although there was variability among proteomes, overall 20% of these proteins were found to be readily amenable to NMR structure determination. NMR sample preparation was centralized in one facility, and a distributive approach was used for NMR data collection and analysis. Twelve structures are reported here as part of this approach, which allowed us to infer putative functions for several conserved hypothetical proteins.
About this Structure
1RYJ is a Single protein structure of sequence from Methanothermococcus thermolithotrophicus. Full crystallographic information is available from OCA.
Reference
An NMR approach to structural proteomics., Yee A, Chang X, Pineda-Lucena A, Wu B, Semesi A, Le B, Ramelot T, Lee GM, Bhattacharyya S, Gutierrez P, Denisov A, Lee CH, Cort JR, Kozlov G, Liao J, Finak G, Chen L, Wishart D, Lee W, McIntosh LP, Gehring K, Kennedy MA, Edwards AM, Arrowsmith CH, Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):1825-30. PMID:11854485
Page seeded by OCA on Thu Feb 21 14:55:55 2008
Categories: Methanothermococcus thermolithotrophicus | Single protein | Arrowsmith, C H. | Bhattacharyya, S. | Chang, X. | Chen, L. | Cort, J R. | Denisov, A. | Edwards, A M. | Finak, G. | Gehring, K. | Gutierrez, P. | Kennedy, M A. | Kozlov, G. | Le, B. | Lee, C H. | Lee, G M. | Lee, W. | Liao, J. | McIntosh, L P. | NESG, Northeast Structural Genomics Consortium. | Pineda-Lucena, A. | Ramelot, T. | Semesi, A. | Wishart, D. | Wu, B. | Yee, A. | Beta/alpha protein | Nesg | Northeast structural genomics consortium | Ocsp | Protein structure initiative | Psi | Structural genomics
