1rz2
From Proteopedia
(New page: 200px<br /><applet load="1rz2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rz2, resolution 1.60Å" /> '''1.6A crystal structu...) |
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| - | [[Image:1rz2.jpg|left|200px]]<br /><applet load="1rz2" size=" | + | [[Image:1rz2.jpg|left|200px]]<br /><applet load="1rz2" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1rz2, resolution 1.60Å" /> | caption="1rz2, resolution 1.60Å" /> | ||
'''1.6A crystal structure of the protein BA4783/Q81L49 (similar to sortase B) from Bacillus anthracis.'''<br /> | '''1.6A crystal structure of the protein BA4783/Q81L49 (similar to sortase B) from Bacillus anthracis.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Surface proteins attached by sortases to the cell wall envelope of | + | Surface proteins attached by sortases to the cell wall envelope of bacterial pathogens play important roles during infection. Sorting and attachment of these proteins is directed by C-terminal signals. Sortase B of S. aureus recognizes a motif NPQTN, cleaves the polypeptide after the Thr residue, and attaches the protein to pentaglycine cross-bridges. Sortase B of B. anthracis is thought to recognize the NPKTG motif, and attaches surface proteins to m-diaminopimelic acid cross-bridges. We have determined crystal structure of sortase B from B. anthracis and S. aureus at 1.6 and 2.0 A resolutions, respectively. These structures show a beta-barrel fold with alpha-helical elements on its outside, a structure thus far exclusive to the sortase family. A putative active site located on the edge of the beta-barrel is comprised of a Cys-His-Asp catalytic triad and presumably faces the bacterial cell surface. A putative binding site for the sorting signal is located nearby. |
==About this Structure== | ==About this Structure== | ||
| - | 1RZ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis_str._ames Bacillus anthracis str. ames]. Full crystallographic information is available from [http:// | + | 1RZ2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis_str._ames Bacillus anthracis str. ames]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Gornicki, P.]] | [[Category: Gornicki, P.]] | ||
[[Category: Joachimiak, A.]] | [[Category: Joachimiak, A.]] | ||
| - | [[Category: MCSG, Midwest | + | [[Category: MCSG, Midwest Center for Structural Genomics.]] |
[[Category: Wu, R.]] | [[Category: Wu, R.]] | ||
[[Category: Zhang, R.]] | [[Category: Zhang, R.]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:56:07 2008'' |
Revision as of 12:56, 21 February 2008
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1.6A crystal structure of the protein BA4783/Q81L49 (similar to sortase B) from Bacillus anthracis.
Overview
Surface proteins attached by sortases to the cell wall envelope of bacterial pathogens play important roles during infection. Sorting and attachment of these proteins is directed by C-terminal signals. Sortase B of S. aureus recognizes a motif NPQTN, cleaves the polypeptide after the Thr residue, and attaches the protein to pentaglycine cross-bridges. Sortase B of B. anthracis is thought to recognize the NPKTG motif, and attaches surface proteins to m-diaminopimelic acid cross-bridges. We have determined crystal structure of sortase B from B. anthracis and S. aureus at 1.6 and 2.0 A resolutions, respectively. These structures show a beta-barrel fold with alpha-helical elements on its outside, a structure thus far exclusive to the sortase family. A putative active site located on the edge of the beta-barrel is comprised of a Cys-His-Asp catalytic triad and presumably faces the bacterial cell surface. A putative binding site for the sorting signal is located nearby.
About this Structure
1RZ2 is a Single protein structure of sequence from Bacillus anthracis str. ames. Full crystallographic information is available from OCA.
Reference
Structures of sortase B from Staphylococcus aureus and Bacillus anthracis reveal catalytic amino acid triad in the active site., Zhang R, Wu R, Joachimiak G, Mazmanian SK, Missiakas DM, Gornicki P, Schneewind O, Joachimiak A, Structure. 2004 Jul;12(7):1147-56. PMID:15242591
Page seeded by OCA on Thu Feb 21 14:56:07 2008
Categories: Bacillus anthracis str. ames | Single protein | Gornicki, P. | Joachimiak, A. | MCSG, Midwest Center for Structural Genomics. | Wu, R. | Zhang, R. | B. anthracis | Mcsg | Midwest center for structural genomics | Protein structure initiative | Psi | Sortase b protein | Structural genomics
