1s0u
From Proteopedia
(New page: 200px<br /><applet load="1s0u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s0u, resolution 2.4Å" /> '''eIF2gamma apo'''<br /...) |
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| - | [[Image:1s0u.jpg|left|200px]]<br /><applet load="1s0u" size=" | + | [[Image:1s0u.jpg|left|200px]]<br /><applet load="1s0u" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1s0u, resolution 2.4Å" /> | caption="1s0u, resolution 2.4Å" /> | ||
'''eIF2gamma apo'''<br /> | '''eIF2gamma apo'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The x-ray structure of the gamma-subunit of the heterotrimeric translation | + | The x-ray structure of the gamma-subunit of the heterotrimeric translation initiation factor eIF2 has been determined to 2.4-A resolution. eIF2 is a GTPase that delivers the initiator Met-tRNA to the P site on the small ribosomal subunit during a rate-limiting initiation step in translation. The structure of eIF2gamma closely resembles that of EF1A.GTP, consisting of an N-terminal G domain followed by two beta-barrels arranged in a closed configuration with domain II packed against the G domain in the vicinity of the Switch regions. The G domain of eIF2gamma has an unusual zinc ribbon motif, not previously found in other GTPases. Structure-based site-directed mutagenesis was used to identify two adjacent features on the surface of eIF2gamma that bind the alpha-subunit and Met-tRNA(i)(Met), respectively. These structural, biochemical, and genetic results provide new insights into eIF2 ternary complex assembly. |
==About this Structure== | ==About this Structure== | ||
| - | 1S0U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1S0U is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S0U OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Alone, P.]] | [[Category: Alone, P.]] | ||
| - | [[Category: Burley, S | + | [[Category: Burley, S K.]] |
[[Category: Cao, C.]] | [[Category: Cao, C.]] | ||
| - | [[Category: Dever, T | + | [[Category: Dever, T E.]] |
[[Category: Roll-Mecak, A.]] | [[Category: Roll-Mecak, A.]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
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[[Category: trna]] | [[Category: trna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:56:42 2008'' |
Revision as of 12:56, 21 February 2008
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eIF2gamma apo
Overview
The x-ray structure of the gamma-subunit of the heterotrimeric translation initiation factor eIF2 has been determined to 2.4-A resolution. eIF2 is a GTPase that delivers the initiator Met-tRNA to the P site on the small ribosomal subunit during a rate-limiting initiation step in translation. The structure of eIF2gamma closely resembles that of EF1A.GTP, consisting of an N-terminal G domain followed by two beta-barrels arranged in a closed configuration with domain II packed against the G domain in the vicinity of the Switch regions. The G domain of eIF2gamma has an unusual zinc ribbon motif, not previously found in other GTPases. Structure-based site-directed mutagenesis was used to identify two adjacent features on the surface of eIF2gamma that bind the alpha-subunit and Met-tRNA(i)(Met), respectively. These structural, biochemical, and genetic results provide new insights into eIF2 ternary complex assembly.
About this Structure
1S0U is a Single protein structure of sequence from Methanocaldococcus jannaschii with as ligand. Full crystallographic information is available from OCA.
Reference
X-ray structure of translation initiation factor eIF2gamma: implications for tRNA and eIF2alpha binding., Roll-Mecak A, Alone P, Cao C, Dever TE, Burley SK, J Biol Chem. 2004 Mar 12;279(11):10634-42. Epub 2003 Dec 19. PMID:14688270
Page seeded by OCA on Thu Feb 21 14:56:42 2008
Categories: Methanocaldococcus jannaschii | Single protein | Alone, P. | Burley, S K. | Cao, C. | Dever, T E. | Roll-Mecak, A. | ZN | Ef-1a | Eif2 | Gtpase | Translation initiation | Trna
