1s1h
From Proteopedia
(New page: 200px<br /><applet load="1s1h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s1h" /> '''Structure of the ribosomal 80S-eEF2-sordarin...) |
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'''Structure of the ribosomal 80S-eEF2-sordarin complex from yeast obtained by docking atomic models for RNA and protein components into a 11.7 A cryo-EM map. This file, 1S1H, Contains 40S subunit. The 60S Ribosomal Subunit Is In File 1S1I.'''<br /> | '''Structure of the ribosomal 80S-eEF2-sordarin complex from yeast obtained by docking atomic models for RNA and protein components into a 11.7 A cryo-EM map. This file, 1S1H, Contains 40S subunit. The 60S Ribosomal Subunit Is In File 1S1I.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | An 11.7-A-resolution cryo-EM map of the yeast 80S.eEF2 complex in the | + | An 11.7-A-resolution cryo-EM map of the yeast 80S.eEF2 complex in the presence of the antibiotic sordarin was interpreted in molecular terms, revealing large conformational changes within eEF2 and the 80S ribosome, including a rearrangement of the functionally important ribosomal intersubunit bridges. Sordarin positions domain III of eEF2 so that it can interact with the sarcin-ricin loop of 25S rRNA and protein rpS23 (S12p). This particular conformation explains the inhibitory action of sordarin and suggests that eEF2 is stalled on the 80S ribosome in a conformation that has similarities with the GTPase activation state. A ratchet-like subunit rearrangement (RSR) occurs in the 80S.eEF2.sordarin complex that, in contrast to Escherichia coli 70S ribosomes, is also present in vacant 80S ribosomes. A model is suggested, according to which the RSR is part of a mechanism for moving the tRNAs during the translocation reaction. |
==About this Structure== | ==About this Structure== | ||
| - | 1S1H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http:// | + | 1S1H is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S1H OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Andersen, G | + | [[Category: Andersen, G R.]] |
| - | [[Category: Ballesta, J | + | [[Category: Ballesta, J P.G.]] |
[[Category: Beckmann, R.]] | [[Category: Beckmann, R.]] | ||
[[Category: Frank, J.]] | [[Category: Frank, J.]] | ||
| - | [[Category: Gomez-Lorenzo, M | + | [[Category: Gomez-Lorenzo, M G.]] |
| - | [[Category: Grassucci, R | + | [[Category: Grassucci, R A.]] |
[[Category: Jorgensen, R.]] | [[Category: Jorgensen, R.]] | ||
| - | [[Category: Penczek, P | + | [[Category: Penczek, P A.]] |
| - | [[Category: Spahn, C | + | [[Category: Spahn, C M.]] |
[[Category: 40s ribosomal subunit]] | [[Category: 40s ribosomal subunit]] | ||
[[Category: 80s ribosome]] | [[Category: 80s ribosome]] | ||
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[[Category: trna translocation]] | [[Category: trna translocation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:56:52 2008'' |
Revision as of 12:56, 21 February 2008
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Structure of the ribosomal 80S-eEF2-sordarin complex from yeast obtained by docking atomic models for RNA and protein components into a 11.7 A cryo-EM map. This file, 1S1H, Contains 40S subunit. The 60S Ribosomal Subunit Is In File 1S1I.
Overview
An 11.7-A-resolution cryo-EM map of the yeast 80S.eEF2 complex in the presence of the antibiotic sordarin was interpreted in molecular terms, revealing large conformational changes within eEF2 and the 80S ribosome, including a rearrangement of the functionally important ribosomal intersubunit bridges. Sordarin positions domain III of eEF2 so that it can interact with the sarcin-ricin loop of 25S rRNA and protein rpS23 (S12p). This particular conformation explains the inhibitory action of sordarin and suggests that eEF2 is stalled on the 80S ribosome in a conformation that has similarities with the GTPase activation state. A ratchet-like subunit rearrangement (RSR) occurs in the 80S.eEF2.sordarin complex that, in contrast to Escherichia coli 70S ribosomes, is also present in vacant 80S ribosomes. A model is suggested, according to which the RSR is part of a mechanism for moving the tRNAs during the translocation reaction.
About this Structure
1S1H is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Domain movements of elongation factor eEF2 and the eukaryotic 80S ribosome facilitate tRNA translocation., Spahn CM, Gomez-Lorenzo MG, Grassucci RA, Jorgensen R, Andersen GR, Beckmann R, Penczek PA, Ballesta JP, Frank J, EMBO J. 2004 Mar 10;23(5):1008-19. Epub 2004 Feb 19. PMID:14976550
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