1s32
From Proteopedia
(New page: 200px<br /> <applet load="1s32" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s32, resolution 2.05Å" /> '''Molecular Recogniti...) |
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| - | [[Image:1s32.gif|left|200px]]<br /> | + | [[Image:1s32.gif|left|200px]]<br /><applet load="1s32" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1s32" size=" | + | |
caption="1s32, resolution 2.05Å" /> | caption="1s32, resolution 2.05Å" /> | ||
'''Molecular Recognition of the Nucleosomal 'Supergroove''''<br /> | '''Molecular Recognition of the Nucleosomal 'Supergroove''''<br /> | ||
==Overview== | ==Overview== | ||
| - | Chromatin is the physiological substrate in all processes involving | + | Chromatin is the physiological substrate in all processes involving eukaryotic DNA. By organizing 147 base pairs of DNA into two tight superhelical coils, the nucleosome generates an architecture where DNA regions that are 80 base pairs apart on linear DNA are brought into close proximity, resulting in the formation of DNA "supergrooves." Here, we report the design of a hairpin polyamide dimer that targets one such supergroove. The 2-A crystal structure of the nucleosome-polyamide complex shows that the bivalent "clamp" effectively crosslinks the two gyres of the DNA superhelix, improves positioning of the DNA on the histone octamer, and stabilizes the nucleosome against dissociation. Our findings identify nucleosomal supergrooves as platforms for molecular recognition of condensed eukaryotic DNA. In vivo, supergrooves may foster synergistic protein-protein interactions by bringing two regulatory elements into juxtaposition. Because supergroove formation is independent of the translational position of the DNA on the histone octamer, accurate nucleosome positioning over regulatory elements is not required for supergroove participation in eukaryotic gene regulation. |
==About this Structure== | ==About this Structure== | ||
| - | 1S32 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with MN, CL, IMT and OGG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1S32 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis] with <scene name='pdbligand=MN:'>MN</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=IMT:'>IMT</scene> and <scene name='pdbligand=OGG:'>OGG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S32 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Xenopus laevis]] | [[Category: Xenopus laevis]] | ||
| - | [[Category: Dervan, P | + | [[Category: Dervan, P B.]] |
| - | [[Category: Edayathumangalam, R | + | [[Category: Edayathumangalam, R S.]] |
| - | [[Category: Gottesfeld, J | + | [[Category: Gottesfeld, J M.]] |
[[Category: Luger, K.]] | [[Category: Luger, K.]] | ||
[[Category: Weyermann, P.]] | [[Category: Weyermann, P.]] | ||
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[[Category: pyrrole-imidazole (py-im) hairpin polyamide]] | [[Category: pyrrole-imidazole (py-im) hairpin polyamide]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:57:22 2008'' |
Revision as of 12:57, 21 February 2008
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Molecular Recognition of the Nucleosomal 'Supergroove'
Overview
Chromatin is the physiological substrate in all processes involving eukaryotic DNA. By organizing 147 base pairs of DNA into two tight superhelical coils, the nucleosome generates an architecture where DNA regions that are 80 base pairs apart on linear DNA are brought into close proximity, resulting in the formation of DNA "supergrooves." Here, we report the design of a hairpin polyamide dimer that targets one such supergroove. The 2-A crystal structure of the nucleosome-polyamide complex shows that the bivalent "clamp" effectively crosslinks the two gyres of the DNA superhelix, improves positioning of the DNA on the histone octamer, and stabilizes the nucleosome against dissociation. Our findings identify nucleosomal supergrooves as platforms for molecular recognition of condensed eukaryotic DNA. In vivo, supergrooves may foster synergistic protein-protein interactions by bringing two regulatory elements into juxtaposition. Because supergroove formation is independent of the translational position of the DNA on the histone octamer, accurate nucleosome positioning over regulatory elements is not required for supergroove participation in eukaryotic gene regulation.
About this Structure
1S32 is a Protein complex structure of sequences from Xenopus laevis with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Molecular recognition of the nucleosomal "supergroove"., Edayathumangalam RS, Weyermann P, Gottesfeld JM, Dervan PB, Luger K, Proc Natl Acad Sci U S A. 2004 May 4;101(18):6864-9. Epub 2004 Apr 20. PMID:15100411
Page seeded by OCA on Thu Feb 21 14:57:22 2008
