1s4j
From Proteopedia
(New page: 200px<br /> <applet load="1s4j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s4j" /> '''NMR structure of cross-reactive peptides fr...) |
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'''NMR structure of cross-reactive peptides from Homo sapiens'''<br /> | '''NMR structure of cross-reactive peptides from Homo sapiens'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of peptides corresponding to the C-terminal residues from | + | The structure of peptides corresponding to the C-terminal residues from Trypanosoma cruzi (R13), human (H13) and Leishmania braziliensis (A13) ribosomal proteins were determined using nuclear magnetic resonance. Although there is only one amino acid difference between them, the peptides present distinct structures in solution: R13 adopts a random coil conformation while H13 and A13 form a bend. Interaction of these peptides with polyclonal antibodies from chronic Chagas' disease patients and a monoclonal antibody raised against T. cruzi ribosomal P2beta protein was probed by transferred NOE. The results show that the flexibility of R13 is fundamental for the binding to the antibody. |
==About this Structure== | ==About this Structure== | ||
| - | 1S4J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1S4J is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4J OCA]. |
==Reference== | ==Reference== | ||
Correlation between conformation and antibody binding: NMR structure of cross-reactive peptides from T. cruzi, human and L. braziliensis., Soares MR, Bisch PM, Campos de Carvalho AC, Valente AP, Almeida FC, FEBS Lett. 2004 Feb 27;560(1-3):134-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14988012 14988012] | Correlation between conformation and antibody binding: NMR structure of cross-reactive peptides from T. cruzi, human and L. braziliensis., Soares MR, Bisch PM, Campos de Carvalho AC, Valente AP, Almeida FC, FEBS Lett. 2004 Feb 27;560(1-3):134-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14988012 14988012] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Almeida, F | + | [[Category: Almeida, F C.L.]] |
| - | [[Category: Bisch, P | + | [[Category: Bisch, P M.]] |
| - | [[Category: Carvalho, A | + | [[Category: Carvalho, A C.Campos de.]] |
| - | [[Category: Soares, M | + | [[Category: Soares, M R.]] |
| - | [[Category: Valente, A | + | [[Category: Valente, A P.]] |
[[Category: antigenic peptide]] | [[Category: antigenic peptide]] | ||
[[Category: chagas disease]] | [[Category: chagas disease]] | ||
[[Category: ribosomal p2 protein]] | [[Category: ribosomal p2 protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:57:49 2008'' |
Revision as of 12:57, 21 February 2008
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NMR structure of cross-reactive peptides from Homo sapiens
Overview
The structure of peptides corresponding to the C-terminal residues from Trypanosoma cruzi (R13), human (H13) and Leishmania braziliensis (A13) ribosomal proteins were determined using nuclear magnetic resonance. Although there is only one amino acid difference between them, the peptides present distinct structures in solution: R13 adopts a random coil conformation while H13 and A13 form a bend. Interaction of these peptides with polyclonal antibodies from chronic Chagas' disease patients and a monoclonal antibody raised against T. cruzi ribosomal P2beta protein was probed by transferred NOE. The results show that the flexibility of R13 is fundamental for the binding to the antibody.
About this Structure
1S4J is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Correlation between conformation and antibody binding: NMR structure of cross-reactive peptides from T. cruzi, human and L. braziliensis., Soares MR, Bisch PM, Campos de Carvalho AC, Valente AP, Almeida FC, FEBS Lett. 2004 Feb 27;560(1-3):134-40. PMID:14988012
Page seeded by OCA on Thu Feb 21 14:57:49 2008
