1s7f
From Proteopedia
(New page: 200px<br /><applet load="1s7f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s7f, resolution 2.0Å" /> '''RimL- Ribosomal L7/L1...) |
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| - | [[Image:1s7f.gif|left|200px]]<br /><applet load="1s7f" size=" | + | [[Image:1s7f.gif|left|200px]]<br /><applet load="1s7f" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1s7f, resolution 2.0Å" /> | caption="1s7f, resolution 2.0Å" /> | ||
'''RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase crystal form I (apo)'''<br /> | '''RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase crystal form I (apo)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | RimL is responsible for converting the prokaryotic ribosomal protein from | + | RimL is responsible for converting the prokaryotic ribosomal protein from L12 to L7 by acetylation of its N-terminal amino group. We demonstrate that purified RimL is capable of posttranslationally acetylating L12, exhibiting a V(max) of 21 min(-1). We have also determined the apostructure of RimL from Salmonella typhimurium and its complex with coenzyme A, revealing a homodimeric oligomer with structural similarity to other Gcn5-related N-acetyltransferase superfamily members. A large central trough located at the dimer interface provides sufficient room to bind both L12 N-terminal helices. Structural and biochemical analysis indicates that RimL proceeds by single-step transfer rather than a covalent-enzyme intermediate. This is the first structure of a Gcn5-related N-acetyltransferase family member with demonstrated activity toward a protein N(alpha)-amino group and is a first step toward understanding the molecular basis for N(alpha)acetylation and its function in cellular regulation. |
==About this Structure== | ==About this Structure== | ||
| - | 1S7F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium_lt2 Salmonella typhimurium lt2] with CL and MLA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1S7F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium_lt2 Salmonella typhimurium lt2] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=MLA:'>MLA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7F OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Salmonella typhimurium lt2]] | [[Category: Salmonella typhimurium lt2]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Blanchard, J | + | [[Category: Blanchard, J S.]] |
| - | [[Category: Carvalho, L | + | [[Category: Carvalho, L P.de.]] |
| - | [[Category: Roderick, S | + | [[Category: Roderick, S L.]] |
| - | [[Category: Vetting, M | + | [[Category: Vetting, M W.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: MLA]] | [[Category: MLA]] | ||
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[[Category: l7/l12]] | [[Category: l7/l12]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:58:44 2008'' |
Revision as of 12:58, 21 February 2008
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RimL- Ribosomal L7/L12 alpha-N-protein acetyltransferase crystal form I (apo)
Overview
RimL is responsible for converting the prokaryotic ribosomal protein from L12 to L7 by acetylation of its N-terminal amino group. We demonstrate that purified RimL is capable of posttranslationally acetylating L12, exhibiting a V(max) of 21 min(-1). We have also determined the apostructure of RimL from Salmonella typhimurium and its complex with coenzyme A, revealing a homodimeric oligomer with structural similarity to other Gcn5-related N-acetyltransferase superfamily members. A large central trough located at the dimer interface provides sufficient room to bind both L12 N-terminal helices. Structural and biochemical analysis indicates that RimL proceeds by single-step transfer rather than a covalent-enzyme intermediate. This is the first structure of a Gcn5-related N-acetyltransferase family member with demonstrated activity toward a protein N(alpha)-amino group and is a first step toward understanding the molecular basis for N(alpha)acetylation and its function in cellular regulation.
About this Structure
1S7F is a Single protein structure of sequence from Salmonella typhimurium lt2 with and as ligands. Full crystallographic information is available from OCA.
Reference
A novel dimeric structure of the RimL Nalpha-acetyltransferase from Salmonella typhimurium., Vetting MW, de Carvalho LP, Roderick SL, Blanchard JS, J Biol Chem. 2005 Jun 10;280(23):22108-14. Epub 2005 Apr 6. PMID:15817456
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