This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1e5b
From Proteopedia
| Line 25: | Line 25: | ||
[[Category: xylanase]] | [[Category: xylanase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 15:06:00 2007'' |
Revision as of 13:01, 30 October 2007
|
INTERNAL XYLAN BINDING DOMAIN FROM C. FIMI XYN10A, R262G MUTANT
Overview
The interactions of proteins with polysaccharides play a key role in the, microbial hydrolysis of cellulose and xylan, the most abundant organic, molecules in the biosphere, and are thus pivotal to the recycling of, photosynthetically fixed carbon. Enzymes that attack these recalcitrant, polymers have a modular structure comprising catalytic modules and, non-catalytic carbohydrate-binding modules (CBMs). The largest prokaryotic, CBM family, CBM2, contains members that bind cellulose (CBM2a) and xylan, (CBM2b), respectively. A possible explanation for the different ligand, specificity of CBM2b is that one of the surface tryptophans involved in, the protein-carbohydrate interaction is rotated by 90 degrees compared, with its position in CBM2a (thus matching the structure of the binding, ... [(full description)]
About this Structure
1E5B is a [Single protein] structure of sequence from [Cellulomonas fimi]. Active as [Endo-1,4-beta-xylanase], with EC number [3.2.1.8]. Structure known Active Site: XBD. Full crystallographic information is available from [OCA].
Reference
The structural basis for the ligand specificity of family 2 carbohydrate-binding modules., Simpson PJ, Xie H, Bolam DN, Gilbert HJ, Williamson MP, J Biol Chem. 2000 Dec 29;275(52):41137-42. PMID:10973978
Page seeded by OCA on Tue Oct 30 15:06:00 2007
