1s8f
From Proteopedia
(New page: 200px<br /><applet load="1s8f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s8f, resolution 1.77Å" /> '''Crystal structure of...) |
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| - | [[Image:1s8f.gif|left|200px]]<br /><applet load="1s8f" size=" | + | [[Image:1s8f.gif|left|200px]]<br /><applet load="1s8f" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1s8f, resolution 1.77Å" /> | caption="1s8f, resolution 1.77Å" /> | ||
'''Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II'''<br /> | '''Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The small GTPase Rab9 is an essential regulator of vesicular transport | + | The small GTPase Rab9 is an essential regulator of vesicular transport from the late endosome to the trans-Golgi network, as monitored by the redirection of the mannose-6-phosphate receptors. The crystal structure of Rab9 complexed to GDP, Mg(2+), and Sr(2+) reveals a unique dimer formed by an intermolecular beta-sheet that buries the switch I regions. Surface area and shape complementarity calculations suggest that Rab9 dimers can form an inactive, membrane-bound pool of Rab9 . GDP that is independent of GDI. Mg(2+)-bound Rab9 represents an inactive state, but Sr(2+)-bound Rab9 . GDP displays activated switch region conformations, mimicking those of the GTP state. A hydrophobic tetrad is formed resembling an effector-discriminating epitope found only in GTP-bound Rab proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1S8F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] with SR, MG, CL, GDP and BEZ as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1S8F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] with <scene name='pdbligand=SR:'>SR</scene>, <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=GDP:'>GDP</scene> and <scene name='pdbligand=BEZ:'>BEZ</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S8F OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Canis lupus familiaris]] | [[Category: Canis lupus familiaris]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Rudolph, M | + | [[Category: Rudolph, M G.]] |
| - | [[Category: Wittmann, J | + | [[Category: Wittmann, J G.]] |
[[Category: BEZ]] | [[Category: BEZ]] | ||
[[Category: CL]] | [[Category: CL]] | ||
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[[Category: vesicular trafficking]] | [[Category: vesicular trafficking]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:02 2008'' |
Revision as of 12:59, 21 February 2008
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Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II
Overview
The small GTPase Rab9 is an essential regulator of vesicular transport from the late endosome to the trans-Golgi network, as monitored by the redirection of the mannose-6-phosphate receptors. The crystal structure of Rab9 complexed to GDP, Mg(2+), and Sr(2+) reveals a unique dimer formed by an intermolecular beta-sheet that buries the switch I regions. Surface area and shape complementarity calculations suggest that Rab9 dimers can form an inactive, membrane-bound pool of Rab9 . GDP that is independent of GDI. Mg(2+)-bound Rab9 represents an inactive state, but Sr(2+)-bound Rab9 . GDP displays activated switch region conformations, mimicking those of the GTP state. A hydrophobic tetrad is formed resembling an effector-discriminating epitope found only in GTP-bound Rab proteins.
About this Structure
1S8F is a Single protein structure of sequence from Canis lupus familiaris with , , , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of Rab9 complexed to GDP reveals a dimer with an active conformation of switch II., Wittmann JG, Rudolph MG, FEBS Lett. 2004 Jun 18;568(1-3):23-9. PMID:15196914
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