1s9b

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(New page: 200px<br /><applet load="1s9b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s9b, resolution 2.81&Aring;" /> '''Crystal Structure An...)
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[[Image:1s9b.gif|left|200px]]<br /><applet load="1s9b" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1s9b.gif|left|200px]]<br /><applet load="1s9b" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1s9b, resolution 2.81&Aring;" />
caption="1s9b, resolution 2.81&Aring;" />
'''Crystal Structure Analysis of the B-DNA GAATTCG'''<br />
'''Crystal Structure Analysis of the B-DNA GAATTCG'''<br />
==Overview==
==Overview==
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DNA oligonucleotides can be used in order to assemble highly structured, materials. Oligonucleotides with sticky ends can form long linear, structures, whereas branching is required to form two- and, three-dimensional nanostructures. In this paper, we show that when Ni(2+), is attached to the N7 atom of guanine, it can also act as a branching, point. Thus, we have found that the heptanucleotide d(GAATTCG) can, assemble into long linear duplex structures, which cross in space to, generate a cubic structure. The three-dimensional arrays are stabilized by, phosphate-Ni(2+)-guanine interactions. For the first time, the, crystallization of a B form DNA oligonucleotide in a cubic system is, reported, space group I23. Large solvent cavities are found among the DNA, duplexes.
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DNA oligonucleotides can be used in order to assemble highly structured materials. Oligonucleotides with sticky ends can form long linear structures, whereas branching is required to form two- and three-dimensional nanostructures. In this paper, we show that when Ni(2+) is attached to the N7 atom of guanine, it can also act as a branching point. Thus, we have found that the heptanucleotide d(GAATTCG) can assemble into long linear duplex structures, which cross in space to generate a cubic structure. The three-dimensional arrays are stabilized by phosphate-Ni(2+)-guanine interactions. For the first time, the crystallization of a B form DNA oligonucleotide in a cubic system is reported, space group I23. Large solvent cavities are found among the DNA duplexes.
==About this Structure==
==About this Structure==
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1S9B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with NI as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1S9B OCA].
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1S9B is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NI:'>NI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9B OCA].
==Reference==
==Reference==
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[[Category: Protein complex]]
[[Category: Protein complex]]
[[Category: Gouyette, C.]]
[[Category: Gouyette, C.]]
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[[Category: Subirana, J.A.]]
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[[Category: Subirana, J A.]]
[[Category: Uson, I.]]
[[Category: Uson, I.]]
[[Category: Valls, N.]]
[[Category: Valls, N.]]
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[[Category: nickel]]
[[Category: nickel]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 25 00:06:24 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:10 2008''

Revision as of 12:59, 21 February 2008

Image:1s9b.gif

1s9b, resolution 2.81Å

Drag the structure with the mouse to rotate

Crystal Structure Analysis of the B-DNA GAATTCG

Overview

DNA oligonucleotides can be used in order to assemble highly structured materials. Oligonucleotides with sticky ends can form long linear structures, whereas branching is required to form two- and three-dimensional nanostructures. In this paper, we show that when Ni(2+) is attached to the N7 atom of guanine, it can also act as a branching point. Thus, we have found that the heptanucleotide d(GAATTCG) can assemble into long linear duplex structures, which cross in space to generate a cubic structure. The three-dimensional arrays are stabilized by phosphate-Ni(2+)-guanine interactions. For the first time, the crystallization of a B form DNA oligonucleotide in a cubic system is reported, space group I23. Large solvent cavities are found among the DNA duplexes.

About this Structure

1S9B is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.

Reference

A cubic arrangement of DNA double helices based on nickel-guanine interactions., Valls N, Uson I, Gouyette C, Subirana JA, J Am Chem Soc. 2004 Jun 30;126(25):7812-6. PMID:15212528

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