1s8n
From Proteopedia
(New page: 200px<br /><applet load="1s8n" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s8n, resolution 1.482Å" /> '''Crystal structure o...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1s8n.gif|left|200px]]<br /><applet load="1s8n" size=" | + | [[Image:1s8n.gif|left|200px]]<br /><applet load="1s8n" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1s8n, resolution 1.482Å" /> | caption="1s8n, resolution 1.482Å" /> | ||
'''Crystal structure of Rv1626 from Mycobacterium tuberculosis'''<br /> | '''Crystal structure of Rv1626 from Mycobacterium tuberculosis'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We describe the crystal structure of Rv1626 from Mycobacterium | + | We describe the crystal structure of Rv1626 from Mycobacterium tuberculosis at 1.48 A resolution and the corresponding solution structure determined from small angle X-ray scattering. The N-terminal domain shows structural homology to the receiver domains found in bacterial two-component systems. The C-terminal domain has high structural homology to a recently discovered RNA binding domain involved in transcriptional antitermination. The molecule in solution was found to be monomeric as it is in the crystal, but in solution it undergoes a conformational change that is triggered by changes in ionic strength. This is the first structure that links the phosphorylation cascade of the two-component systems with the antitermination event in the transcriptional machinery. Rv1626 belongs to a family of proteins, which we propose calling phosphorylation-dependent transcriptional antitermination regulators, so far only found in bacteria, and includes NasT, a protein from the assimilatory nitrate/nitrite reductase operon of Azetobacter vinelandii. |
==About this Structure== | ==About this Structure== | ||
| - | 1S8N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv] with AZI as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1S8N is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_h37rv Mycobacterium tuberculosis h37rv] with <scene name='pdbligand=AZI:'>AZI</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S8N OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Feng, V.]] | [[Category: Feng, V.]] | ||
| - | [[Category: Morth, J | + | [[Category: Morth, J P.]] |
| - | [[Category: Perry, L | + | [[Category: Perry, L J.]] |
| - | [[Category: Svergun, D | + | [[Category: Svergun, D I.]] |
| - | [[Category: TBSGC, TB | + | [[Category: TBSGC, TB Structural Genomics Consortium.]] |
| - | [[Category: Tucker, P | + | [[Category: Tucker, P A.]] |
[[Category: AZI]] | [[Category: AZI]] | ||
[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
| Line 29: | Line 29: | ||
[[Category: two component system]] | [[Category: two component system]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:59:07 2008'' |
Revision as of 12:59, 21 February 2008
|
Crystal structure of Rv1626 from Mycobacterium tuberculosis
Overview
We describe the crystal structure of Rv1626 from Mycobacterium tuberculosis at 1.48 A resolution and the corresponding solution structure determined from small angle X-ray scattering. The N-terminal domain shows structural homology to the receiver domains found in bacterial two-component systems. The C-terminal domain has high structural homology to a recently discovered RNA binding domain involved in transcriptional antitermination. The molecule in solution was found to be monomeric as it is in the crystal, but in solution it undergoes a conformational change that is triggered by changes in ionic strength. This is the first structure that links the phosphorylation cascade of the two-component systems with the antitermination event in the transcriptional machinery. Rv1626 belongs to a family of proteins, which we propose calling phosphorylation-dependent transcriptional antitermination regulators, so far only found in bacteria, and includes NasT, a protein from the assimilatory nitrate/nitrite reductase operon of Azetobacter vinelandii.
About this Structure
1S8N is a Single protein structure of sequence from Mycobacterium tuberculosis h37rv with as ligand. Full crystallographic information is available from OCA.
Reference
The crystal and solution structure of a putative transcriptional antiterminator from Mycobacterium tuberculosis., Morth JP, Feng V, Perry LJ, Svergun DI, Tucker PA, Structure. 2004 Sep;12(9):1595-605. PMID:15341725
Page seeded by OCA on Thu Feb 21 14:59:07 2008
Categories: Mycobacterium tuberculosis h37rv | Single protein | Feng, V. | Morth, J P. | Perry, L J. | Svergun, D I. | TBSGC, TB Structural Genomics Consortium. | Tucker, P A. | AZI | Protein structure initiative | Psi | Rv1626 | Structural genomics | Tb structural genomics consortium | Tbsgc | Transcriptional antiterminator | Two component system
