1se0

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(New page: 200px<br /><applet load="1se0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1se0, resolution 1.75&Aring;" /> '''Crystal structure of...)
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[[Image:1se0.gif|left|200px]]<br /><applet load="1se0" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1se0.gif|left|200px]]<br /><applet load="1se0" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1se0, resolution 1.75&Aring;" />
caption="1se0, resolution 1.75&Aring;" />
'''Crystal structure of DIAP1 BIR1 bound to a Grim peptide'''<br />
'''Crystal structure of DIAP1 BIR1 bound to a Grim peptide'''<br />
==Overview==
==Overview==
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The Drosophila melanogaster inhibitor of apoptosis protein DIAP1, suppresses apoptosis in part through inhibition of the effector caspase, DrICE. The pro-death proteins Reaper, Hid and Grim (RHG) induce apoptosis, by antagonizing DIAP1 function. However, the underlying molecular, mechanisms remain unknown. Here we demonstrate that DIAP1 directly, inhibits the catalytic activity of DrICE through its BIR1 domain and this, inhibition is countered effectively by the RHG proteins. Inhibition of, DrICE by DIAP1 occurs only after the cleavage of its N-terminal 20 amino, acids and involves a conserved surface groove on BIR1. Crystal structures, of BIR1 bound to the RHG peptides show that the RHG proteins use their, N-terminal IAP-binding motifs to bind to the same surface groove, hence, relieving DIAP1-mediated inhibition of DrICE. These studies define novel, molecular mechanisms for the inhibition and activation of a representative, D. melanogaster effector caspase.
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The Drosophila melanogaster inhibitor of apoptosis protein DIAP1 suppresses apoptosis in part through inhibition of the effector caspase DrICE. The pro-death proteins Reaper, Hid and Grim (RHG) induce apoptosis by antagonizing DIAP1 function. However, the underlying molecular mechanisms remain unknown. Here we demonstrate that DIAP1 directly inhibits the catalytic activity of DrICE through its BIR1 domain and this inhibition is countered effectively by the RHG proteins. Inhibition of DrICE by DIAP1 occurs only after the cleavage of its N-terminal 20 amino acids and involves a conserved surface groove on BIR1. Crystal structures of BIR1 bound to the RHG peptides show that the RHG proteins use their N-terminal IAP-binding motifs to bind to the same surface groove, hence relieving DIAP1-mediated inhibition of DrICE. These studies define novel molecular mechanisms for the inhibition and activation of a representative D. melanogaster effector caspase.
==About this Structure==
==About this Structure==
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1SE0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SE0 OCA].
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1SE0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SE0 OCA].
==Reference==
==Reference==
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[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Shi, Y.]]
[[Category: Shi, Y.]]
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[[Category: Wu, J.W.]]
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[[Category: Wu, J W.]]
[[Category: Yan, N.]]
[[Category: Yan, N.]]
[[Category: ZN]]
[[Category: ZN]]
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[[Category: iap]]
[[Category: iap]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:18:39 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:00:30 2008''

Revision as of 13:00, 21 February 2008

Image:1se0.gif

1se0, resolution 1.75Å

Drag the structure with the mouse to rotate

Crystal structure of DIAP1 BIR1 bound to a Grim peptide

Overview

The Drosophila melanogaster inhibitor of apoptosis protein DIAP1 suppresses apoptosis in part through inhibition of the effector caspase DrICE. The pro-death proteins Reaper, Hid and Grim (RHG) induce apoptosis by antagonizing DIAP1 function. However, the underlying molecular mechanisms remain unknown. Here we demonstrate that DIAP1 directly inhibits the catalytic activity of DrICE through its BIR1 domain and this inhibition is countered effectively by the RHG proteins. Inhibition of DrICE by DIAP1 occurs only after the cleavage of its N-terminal 20 amino acids and involves a conserved surface groove on BIR1. Crystal structures of BIR1 bound to the RHG peptides show that the RHG proteins use their N-terminal IAP-binding motifs to bind to the same surface groove, hence relieving DIAP1-mediated inhibition of DrICE. These studies define novel molecular mechanisms for the inhibition and activation of a representative D. melanogaster effector caspase.

About this Structure

1SE0 is a Single protein structure of sequence from Drosophila melanogaster with as ligand. Full crystallographic information is available from OCA.

Reference

Molecular mechanisms of DrICE inhibition by DIAP1 and removal of inhibition by Reaper, Hid and Grim., Yan N, Wu JW, Chai J, Li W, Shi Y, Nat Struct Mol Biol. 2004 May;11(5):420-8. Epub 2004 Apr 25. PMID:15107838

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