1sgl
From Proteopedia
(New page: 200px<br /><applet load="1sgl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sgl, resolution 2.20Å" /> '''The three-dimensiona...) |
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| - | [[Image:1sgl.gif|left|200px]]<br /><applet load="1sgl" size=" | + | [[Image:1sgl.gif|left|200px]]<br /><applet load="1sgl" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1sgl, resolution 2.20Å" /> | caption="1sgl, resolution 2.20Å" /> | ||
'''The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease'''<br /> | '''The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Trichomaglin is a protein isolated from root tuber of the plant Maganlin | + | Trichomaglin is a protein isolated from root tuber of the plant Maganlin (Trichosanthes Lepiniate, Cucurbitaceae). The crystal structure of trichomaglin has been determined by multiple-isomorphous replacement and refined at 2.2 A resolution. The X-ray sequence was established, based on electron density combined with the experimentally determined N-terminal sequence, and the sequence information derived from mass spectroscopic analysis. X-ray sequence-based homolog search and the three-dimensional structure reveal that trichomaglin is a novel S-like RNase, which was confirmed by biological assay. Trichomaglin molecule contains an additional beta sheet in the HV(b) region, compared with the known plant RNase structures. Fourteen cystein residues form seven disulfide bridges, more than those in the other known structures of S- and S-like RNases. His43 and His105 are expected to be the catalytic acid and base, respectively. Four hydrosulfate ions are bound in the active site pocket, three of them mimicking the substrate binding sites. |
==About this Structure== | ==About this Structure== | ||
| - | 1SGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Trichosanthes_lepiniana Trichosanthes lepiniana] with SOH as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1SGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Trichosanthes_lepiniana Trichosanthes lepiniana] with <scene name='pdbligand=SOH:'>SOH</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SGL OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Trichosanthes lepiniana]] | [[Category: Trichosanthes lepiniana]] | ||
| - | [[Category: Blackstock, W | + | [[Category: Blackstock, W P.]] |
| - | [[Category: Choudhary, J | + | [[Category: Choudhary, J S.]] |
| - | [[Category: Gan, J | + | [[Category: Gan, J H.]] |
| - | [[Category: Liu, W | + | [[Category: Liu, W Y.]] |
[[Category: Wu, J.]] | [[Category: Wu, J.]] | ||
| - | [[Category: Xia, Z | + | [[Category: Xia, Z X.]] |
[[Category: Xu, H.]] | [[Category: Xu, H.]] | ||
[[Category: Yu, L.]] | [[Category: Yu, L.]] | ||
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[[Category: x-ray sequence]] | [[Category: x-ray sequence]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:01:17 2008'' |
Revision as of 13:01, 21 February 2008
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The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease
Overview
Trichomaglin is a protein isolated from root tuber of the plant Maganlin (Trichosanthes Lepiniate, Cucurbitaceae). The crystal structure of trichomaglin has been determined by multiple-isomorphous replacement and refined at 2.2 A resolution. The X-ray sequence was established, based on electron density combined with the experimentally determined N-terminal sequence, and the sequence information derived from mass spectroscopic analysis. X-ray sequence-based homolog search and the three-dimensional structure reveal that trichomaglin is a novel S-like RNase, which was confirmed by biological assay. Trichomaglin molecule contains an additional beta sheet in the HV(b) region, compared with the known plant RNase structures. Fourteen cystein residues form seven disulfide bridges, more than those in the other known structures of S- and S-like RNases. His43 and His105 are expected to be the catalytic acid and base, respectively. Four hydrosulfate ions are bound in the active site pocket, three of them mimicking the substrate binding sites.
About this Structure
1SGL is a Single protein structure of sequence from Trichosanthes lepiniana with as ligand. Full crystallographic information is available from OCA.
Reference
The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease., Gan JH, Yu L, Wu J, Xu H, Choudhary JS, Blackstock WP, Liu WY, Xia ZX, Structure. 2004 Jun;12(6):1015-25. PMID:15274921
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