1sh2
From Proteopedia
(New page: 200px<br /><applet load="1sh2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sh2, resolution 2.30Å" /> '''Crystal Structure of...) |
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| - | [[Image:1sh2.jpg|left|200px]]<br /><applet load="1sh2" size=" | + | [[Image:1sh2.jpg|left|200px]]<br /><applet load="1sh2" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1sh2, resolution 2.30Å" /> | caption="1sh2, resolution 2.30Å" /> | ||
'''Crystal Structure of Norwalk Virus Polymerase (Metal-free, Centered Orthorhombic)'''<br /> | '''Crystal Structure of Norwalk Virus Polymerase (Metal-free, Centered Orthorhombic)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Norwalk virus is a major cause of acute gastroenteritis for which | + | Norwalk virus is a major cause of acute gastroenteritis for which effective treatments are sorely lacking. To provide a basis for the rational design of novel antiviral agents, the main replication enzyme in Norwalk virus, the virally encoded RNA-dependent RNA polymerase (RdRP), has been expressed in an enzymatically active form, and its structure has been crystallographically determined both in the presence and absence of divalent metal cations. Although the overall fold of the enzyme is similar to that seen previously in the RdRP from rabbit hemorrhagic disease virus, the carboxyl terminus, surprisingly, is located in the active site cleft in five independent copies of the protein in three distinct crystal forms. The location of this carboxyl-terminal segment appears to interfere with the binding of double-stranded RNA in the active site cleft and may play a role in the initiation of RNA synthesis or mediate interactions with accessory replication proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1SH2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Norwalk_virus Norwalk virus]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http:// | + | 1SH2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Norwalk_virus Norwalk virus]. Active as [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SH2 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: RNA-directed RNA polymerase]] | [[Category: RNA-directed RNA polymerase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Alonso, J | + | [[Category: Alonso, J M.]] |
| - | [[Category: Boga, J | + | [[Category: Boga, J A.]] |
[[Category: Machin, A.]] | [[Category: Machin, A.]] | ||
| - | [[Category: Ng, K | + | [[Category: Ng, K K.]] |
[[Category: Parra, F.]] | [[Category: Parra, F.]] | ||
[[Category: Pendas-Franco, N.]] | [[Category: Pendas-Franco, N.]] | ||
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[[Category: viral replication]] | [[Category: viral replication]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:01:25 2008'' |
Revision as of 13:01, 21 February 2008
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Crystal Structure of Norwalk Virus Polymerase (Metal-free, Centered Orthorhombic)
Overview
Norwalk virus is a major cause of acute gastroenteritis for which effective treatments are sorely lacking. To provide a basis for the rational design of novel antiviral agents, the main replication enzyme in Norwalk virus, the virally encoded RNA-dependent RNA polymerase (RdRP), has been expressed in an enzymatically active form, and its structure has been crystallographically determined both in the presence and absence of divalent metal cations. Although the overall fold of the enzyme is similar to that seen previously in the RdRP from rabbit hemorrhagic disease virus, the carboxyl terminus, surprisingly, is located in the active site cleft in five independent copies of the protein in three distinct crystal forms. The location of this carboxyl-terminal segment appears to interfere with the binding of double-stranded RNA in the active site cleft and may play a role in the initiation of RNA synthesis or mediate interactions with accessory replication proteins.
About this Structure
1SH2 is a Single protein structure of sequence from Norwalk virus. Active as RNA-directed RNA polymerase, with EC number 2.7.7.48 Full crystallographic information is available from OCA.
Reference
Crystal structure of norwalk virus polymerase reveals the carboxyl terminus in the active site cleft., Ng KK, Pendas-Franco N, Rojo J, Boga JA, Machin A, Alonso JM, Parra F, J Biol Chem. 2004 Apr 16;279(16):16638-45. Epub 2004 Feb 5. PMID:14764591
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