1sig
From Proteopedia
(New page: 200px<br /><applet load="1sig" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sig, resolution 2.6Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1sig.jpg|left|200px]]<br /><applet load="1sig" size=" | + | [[Image:1sig.jpg|left|200px]]<br /><applet load="1sig" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1sig, resolution 2.6Å" /> | caption="1sig, resolution 2.6Å" /> | ||
'''CRYSTAL STRUCTURE OF A SIGMA70 SUBUNIT FRAGMENT FROM ESCHERICHIA COLI RNA POLYMERASE'''<br /> | '''CRYSTAL STRUCTURE OF A SIGMA70 SUBUNIT FRAGMENT FROM ESCHERICHIA COLI RNA POLYMERASE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The 2.6 A crystal structure of a fragment of the sigma 70 promoter | + | The 2.6 A crystal structure of a fragment of the sigma 70 promoter specificity subunit of E. coli RNA polymerase is described. Residues involved in core RNA polymerase binding lie on one face of the structure. On the opposite face, aligned along one helix, are exposed residues that interact with the -10 consensus promoter element (the Pribnow box), including four aromatic residues involved in promoter melting. The structure suggests one way in which DNA interactions may be inhibited in the absence of RNA polymerase and provides a framework for the interpretation of a large number of genetic and biochemical analyses. |
==About this Structure== | ==About this Structure== | ||
| - | 1SIG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1SIG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SIG OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Darst, S | + | [[Category: Darst, S A.]] |
[[Category: Malhotra, A.]] | [[Category: Malhotra, A.]] | ||
[[Category: Severinova, E.]] | [[Category: Severinova, E.]] | ||
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[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:01:49 2008'' |
Revision as of 13:01, 21 February 2008
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CRYSTAL STRUCTURE OF A SIGMA70 SUBUNIT FRAGMENT FROM ESCHERICHIA COLI RNA POLYMERASE
Overview
The 2.6 A crystal structure of a fragment of the sigma 70 promoter specificity subunit of E. coli RNA polymerase is described. Residues involved in core RNA polymerase binding lie on one face of the structure. On the opposite face, aligned along one helix, are exposed residues that interact with the -10 consensus promoter element (the Pribnow box), including four aromatic residues involved in promoter melting. The structure suggests one way in which DNA interactions may be inhibited in the absence of RNA polymerase and provides a framework for the interpretation of a large number of genetic and biochemical analyses.
About this Structure
1SIG is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Crystal structure of a sigma 70 subunit fragment from E. coli RNA polymerase., Malhotra A, Severinova E, Darst SA, Cell. 1996 Oct 4;87(1):127-36. PMID:8858155
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