1sjm
From Proteopedia
(New page: 200px<br /><applet load="1sjm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sjm, resolution 1.40Å" /> '''Nitrite bound copper...) |
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| - | [[Image:1sjm.gif|left|200px]]<br /><applet load="1sjm" size=" | + | [[Image:1sjm.gif|left|200px]]<br /><applet load="1sjm" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1sjm, resolution 1.40Å" /> | caption="1sjm, resolution 1.40Å" /> | ||
'''Nitrite bound copper containing nitrite reductase'''<br /> | '''Nitrite bound copper containing nitrite reductase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A copper-nitrosyl intermediate forms during the catalytic cycle of nitrite | + | A copper-nitrosyl intermediate forms during the catalytic cycle of nitrite reductase, the enzyme that mediates the committed step in bacterial denitrification. The crystal structure of a type 2 copper-nitrosyl complex of nitrite reductase reveals an unprecedented side-on binding mode in which the nitrogen and oxygen atoms are nearly equidistant from the copper cofactor. Comparison of this structure with a refined nitrite-bound crystal structure explains how coordination can change between copper-oxygen and copper-nitrogen during catalysis. The side-on copper-nitrosyl in nitrite reductase expands the possibilities for nitric oxide interactions in copper proteins such as superoxide dismutase and prions. |
==About this Structure== | ==About this Structure== | ||
| - | 1SJM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with CU, NO2, ACT and TRS as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] Full crystallographic information is available from [http:// | + | 1SJM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis] with <scene name='pdbligand=CU:'>CU</scene>, <scene name='pdbligand=NO2:'>NO2</scene>, <scene name='pdbligand=ACT:'>ACT</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Nitrite_reductase_(NO-forming) Nitrite reductase (NO-forming)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.2.1 1.7.2.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SJM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Nitrite reductase (NO-forming)]] | [[Category: Nitrite reductase (NO-forming)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Mauk, A | + | [[Category: Mauk, A G.]] |
| - | [[Category: Murphy, M | + | [[Category: Murphy, M E.P.]] |
| - | [[Category: Rosell, F | + | [[Category: Rosell, F I.]] |
| - | [[Category: Tocheva, E | + | [[Category: Tocheva, E I.]] |
[[Category: ACT]] | [[Category: ACT]] | ||
[[Category: CU]] | [[Category: CU]] | ||
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[[Category: nitrite]] | [[Category: nitrite]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:02:14 2008'' |
Revision as of 13:02, 21 February 2008
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Nitrite bound copper containing nitrite reductase
Overview
A copper-nitrosyl intermediate forms during the catalytic cycle of nitrite reductase, the enzyme that mediates the committed step in bacterial denitrification. The crystal structure of a type 2 copper-nitrosyl complex of nitrite reductase reveals an unprecedented side-on binding mode in which the nitrogen and oxygen atoms are nearly equidistant from the copper cofactor. Comparison of this structure with a refined nitrite-bound crystal structure explains how coordination can change between copper-oxygen and copper-nitrogen during catalysis. The side-on copper-nitrosyl in nitrite reductase expands the possibilities for nitric oxide interactions in copper proteins such as superoxide dismutase and prions.
About this Structure
1SJM is a Single protein structure of sequence from Alcaligenes faecalis with , , and as ligands. Active as Nitrite reductase (NO-forming), with EC number 1.7.2.1 Full crystallographic information is available from OCA.
Reference
Side-on copper-nitrosyl coordination by nitrite reductase., Tocheva EI, Rosell FI, Mauk AG, Murphy ME, Science. 2004 May 7;304(5672):867-70. PMID:15131305
Page seeded by OCA on Thu Feb 21 15:02:14 2008
Categories: Alcaligenes faecalis | Nitrite reductase (NO-forming) | Single protein | Mauk, A G. | Murphy, M E.P. | Rosell, F I. | Tocheva, E I. | ACT | CU | NO2 | TRS | Copper | Nitrite
