1sl7
From Proteopedia
(New page: 200px<br /><applet load="1sl7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sl7, resolution 2.20Å" /> '''Crystal structure of...) |
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| - | [[Image:1sl7.jpg|left|200px]]<br /><applet load="1sl7" size=" | + | [[Image:1sl7.jpg|left|200px]]<br /><applet load="1sl7" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1sl7, resolution 2.20Å" /> | caption="1sl7, resolution 2.20Å" /> | ||
'''Crystal structure of calcium-loaded apo-obelin from Obelia longissima'''<br /> | '''Crystal structure of calcium-loaded apo-obelin from Obelia longissima'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structures of calcium-loaded apo-aequorin and apo-obelin have | + | The crystal structures of calcium-loaded apo-aequorin and apo-obelin have been determined at resolutions 1.7A and 2.2 A, respectively. A calcium ion is observed in each of the three EF-hand loops that have the canonical calcium-binding sequence, and each is coordinated in the characteristic pentagonal bipyramidal configuration. The calcium-loaded apo-protein retain the same compact scaffold and overall fold as the unreacted photoproteins containing the bound substrate, 2-hyroperoxycoelenterazine, and also the same as the Ca2+-discharged obelin bound with product, coleneteramide. Nevertheless, there are easily discerned shifts in both helix and loop regions, and the shifts are not the same between the two proteins. It is suggested that these photoproteins to sense Ca2+ concentration transients and to produce their bioluminescence response on the millisecond timescale. A mechanism of intrastructural transmission of the calcium signal is proposed. |
==About this Structure== | ==About this Structure== | ||
| - | 1SL7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Obelia_longissima Obelia longissima] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1SL7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Obelia_longissima Obelia longissima] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SL7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Deng, L.]] | [[Category: Deng, L.]] | ||
[[Category: Lee, J.]] | [[Category: Lee, J.]] | ||
| - | [[Category: Liu, Z | + | [[Category: Liu, Z J.]] |
| - | [[Category: Markova, S | + | [[Category: Markova, S V.]] |
[[Category: Rose, J.]] | [[Category: Rose, J.]] | ||
| - | [[Category: SECSG, Southeast | + | [[Category: SECSG, Southeast Collaboratory for Structural Genomics.]] |
| - | [[Category: Vysotski, E | + | [[Category: Vysotski, E S.]] |
| - | [[Category: Wang, B | + | [[Category: Wang, B C.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: aequorin]] | [[Category: aequorin]] | ||
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[[Category: structural genomics]] | [[Category: structural genomics]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:02:37 2008'' |
Revision as of 13:02, 21 February 2008
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Crystal structure of calcium-loaded apo-obelin from Obelia longissima
Overview
The crystal structures of calcium-loaded apo-aequorin and apo-obelin have been determined at resolutions 1.7A and 2.2 A, respectively. A calcium ion is observed in each of the three EF-hand loops that have the canonical calcium-binding sequence, and each is coordinated in the characteristic pentagonal bipyramidal configuration. The calcium-loaded apo-protein retain the same compact scaffold and overall fold as the unreacted photoproteins containing the bound substrate, 2-hyroperoxycoelenterazine, and also the same as the Ca2+-discharged obelin bound with product, coleneteramide. Nevertheless, there are easily discerned shifts in both helix and loop regions, and the shifts are not the same between the two proteins. It is suggested that these photoproteins to sense Ca2+ concentration transients and to produce their bioluminescence response on the millisecond timescale. A mechanism of intrastructural transmission of the calcium signal is proposed.
About this Structure
1SL7 is a Single protein structure of sequence from Obelia longissima with as ligand. Full crystallographic information is available from OCA.
Reference
All three Ca2+-binding loops of photoproteins bind calcium ions: the crystal structures of calcium-loaded apo-aequorin and apo-obelin., Deng L, Vysotski ES, Markova SV, Liu ZJ, Lee J, Rose J, Wang BC, Protein Sci. 2005 Mar;14(3):663-75. Epub 2005 Feb 2. PMID:15689515
Page seeded by OCA on Thu Feb 21 15:02:37 2008
Categories: Obelia longissima | Single protein | Deng, L. | Lee, J. | Liu, Z J. | Markova, S V. | Rose, J. | SECSG, Southeast Collaboratory for Structural Genomics. | Vysotski, E S. | Wang, B C. | CA | Aequorin | Bioluminescence | Calcium binding | Ef-hand | Fluorescence | Obelin | Photoprotein | Protein structure initiative | Psi | Secsg | Southeast collaboratory for structural genomics | Structural genomics
