1sls
From Proteopedia
(New page: 200px<br /><applet load="1sls" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sls" /> '''IMMOBILE SLIPPED-LOOP STRUCTURE (SLS) OF DNA...) |
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'''IMMOBILE SLIPPED-LOOP STRUCTURE (SLS) OF DNA HOMODIMER IN SOLUTION, NMR, 9 STRUCTURES'''<br /> | '''IMMOBILE SLIPPED-LOOP STRUCTURE (SLS) OF DNA HOMODIMER IN SOLUTION, NMR, 9 STRUCTURES'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We report a high-resolution NMR structure of a homodimer formed by a | + | We report a high-resolution NMR structure of a homodimer formed by a synthetic 25 residue DNA oligonucleotide GCTCCCATGGTTTTTGTGCACGAGC. This structure presents a novel structural motif for single-stranded nucleic acids, called a pseudosquare knot (PSQ). The oligonucleotide was originally designed to mimic a slipped-loop structure (SLS), another "unusual" DNA structure postulated as an alternative conformation for short direct repeats in double-stranded DNA. The design of the sequence is compatible with both SLS and PSQ structures, both of which possess identical sets of base-paired and unpaired nucleotides but different tertiary folds. We used deuteration of the H8 positions of purines to ascertain that the PSQ is actually formed under the conditions used. The PSQ structure was solved based on homonuclear proton nuclear Overhauser effect data using complete relaxation matrix methods. The structure essentially consists of two side-by-side helices connected by single-stranded loops. Each of the helices is well-defined; however, the relative orientation of the two remains undetermined by the NMR data. The sequences compatible with the PSQ formation are frequent in single-stranded genomes; this structure may play a role as a dimerization motif. |
==About this Structure== | ==About this Structure== | ||
| - | 1SLS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1SLS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SLS OCA]. |
==Reference== | ==Reference== | ||
A pseudosquare knot structure of DNA in solution., Ulyanov NB, Ivanov VI, Minyat EE, Khomyakova EB, Petrova MV, Lesiak K, James TL, Biochemistry. 1998 Sep 15;37(37):12715-26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9737848 9737848] | A pseudosquare knot structure of DNA in solution., Ulyanov NB, Ivanov VI, Minyat EE, Khomyakova EB, Petrova MV, Lesiak K, James TL, Biochemistry. 1998 Sep 15;37(37):12715-26. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9737848 9737848] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Ivanov, V | + | [[Category: Ivanov, V I.]] |
| - | [[Category: James, T | + | [[Category: James, T L.]] |
| - | [[Category: Khomyakova, E | + | [[Category: Khomyakova, E B.]] |
[[Category: Lesiak, K.]] | [[Category: Lesiak, K.]] | ||
| - | [[Category: Minyat, E | + | [[Category: Minyat, E E.]] |
| - | [[Category: Petrova, M | + | [[Category: Petrova, M V.]] |
| - | [[Category: Ulyanov, N | + | [[Category: Ulyanov, N B.]] |
[[Category: deoxyribonucleic acid]] | [[Category: deoxyribonucleic acid]] | ||
[[Category: dna oligonucleotide]] | [[Category: dna oligonucleotide]] | ||
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[[Category: unusual structure]] | [[Category: unusual structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:02:50 2008'' |
Revision as of 13:02, 21 February 2008
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IMMOBILE SLIPPED-LOOP STRUCTURE (SLS) OF DNA HOMODIMER IN SOLUTION, NMR, 9 STRUCTURES
Overview
We report a high-resolution NMR structure of a homodimer formed by a synthetic 25 residue DNA oligonucleotide GCTCCCATGGTTTTTGTGCACGAGC. This structure presents a novel structural motif for single-stranded nucleic acids, called a pseudosquare knot (PSQ). The oligonucleotide was originally designed to mimic a slipped-loop structure (SLS), another "unusual" DNA structure postulated as an alternative conformation for short direct repeats in double-stranded DNA. The design of the sequence is compatible with both SLS and PSQ structures, both of which possess identical sets of base-paired and unpaired nucleotides but different tertiary folds. We used deuteration of the H8 positions of purines to ascertain that the PSQ is actually formed under the conditions used. The PSQ structure was solved based on homonuclear proton nuclear Overhauser effect data using complete relaxation matrix methods. The structure essentially consists of two side-by-side helices connected by single-stranded loops. Each of the helices is well-defined; however, the relative orientation of the two remains undetermined by the NMR data. The sequences compatible with the PSQ formation are frequent in single-stranded genomes; this structure may play a role as a dimerization motif.
About this Structure
1SLS is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A pseudosquare knot structure of DNA in solution., Ulyanov NB, Ivanov VI, Minyat EE, Khomyakova EB, Petrova MV, Lesiak K, James TL, Biochemistry. 1998 Sep 15;37(37):12715-26. PMID:9737848
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