1smz
From Proteopedia
(New page: 200px<br /><applet load="1smz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1smz" /> '''Structure of Transportan in phospholipid bic...) |
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'''Structure of Transportan in phospholipid bicellar solution'''<br /> | '''Structure of Transportan in phospholipid bicellar solution'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Transportan is a chimeric cell-penetrating peptide constructed from the | + | Transportan is a chimeric cell-penetrating peptide constructed from the peptides galanin and mastoparan, which has the ability to internalize living cells carrying a hydrophilic load. In this study, we have determined the NMR solution structure and investigated the position of transportan in neutral bicelles. The structure revealed a well-defined alpha-helix in the C-terminal mastoparan part of the peptide and a weaker tendency to form an alpha-helix in the N-terminal domain. The position of the peptide in relation to the membrane, as studied by adding paramagnetic probes, shows that the peptide lies parallel to, and in the head-group region of the membrane surface. This result is supported by amide proton secondary chemical shifts. |
==About this Structure== | ==About this Structure== | ||
| - | 1SMZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1SMZ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SMZ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: transport protein]] | [[Category: transport protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:03:10 2008'' |
Revision as of 13:03, 21 February 2008
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Structure of Transportan in phospholipid bicellar solution
Overview
Transportan is a chimeric cell-penetrating peptide constructed from the peptides galanin and mastoparan, which has the ability to internalize living cells carrying a hydrophilic load. In this study, we have determined the NMR solution structure and investigated the position of transportan in neutral bicelles. The structure revealed a well-defined alpha-helix in the C-terminal mastoparan part of the peptide and a weaker tendency to form an alpha-helix in the N-terminal domain. The position of the peptide in relation to the membrane, as studied by adding paramagnetic probes, shows that the peptide lies parallel to, and in the head-group region of the membrane surface. This result is supported by amide proton secondary chemical shifts.
About this Structure
1SMZ is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
NMR solution structure and position of transportan in neutral phospholipid bicelles., Barany-Wallje E, Andersson A, Graslund A, Maler L, FEBS Lett. 2004 Jun 4;567(2-3):265-9. PMID:15178334
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