1sqw
From Proteopedia
(New page: 200px<br /> <applet load="1sqw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sqw, resolution 1.9Å" /> '''Crystal structure of...) |
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| - | [[Image:1sqw.gif|left|200px]]<br /> | + | [[Image:1sqw.gif|left|200px]]<br /><applet load="1sqw" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1sqw" size=" | + | |
caption="1sqw, resolution 1.9Å" /> | caption="1sqw, resolution 1.9Å" /> | ||
'''Crystal structure of KD93, a novel protein expressed in the human pro'''<br /> | '''Crystal structure of KD93, a novel protein expressed in the human pro'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of a novel hypothetical protein, KD93, expressed in | + | The crystal structure of a novel hypothetical protein, KD93, expressed in human hematopoietic stem/progenitor cells, was determined at 1.9A resolution using the multiple-wavelength anomalous dispersion (MAD) method. The protein KD93, which is encoded by the open reading frame HSPC031, is a NIP7 homologue and belongs to the UPF0113 family. The structural and functional information for the group of homologues has not yet been determined. Crystallographic analysis revealed that the overall fold of KD93 consists of two interlinked alpha/beta domains. Structure-based homology analysis with DALI revealed that the C domain of KD93 matches the PUA domain of some RNA modification enzymes, especially that of archaeosine tRNA-ribosyltransferase (ArcTGT), which suggests that its possible molecular function is related to RNA binding. The difference between the RNA binding regions of KD93 and ArcTGT in amino acid constitution and surface electrostatic potential indicate that they may have different RNA binding modes. The N domain of KD93 is a unique structure with no obvious similarity to other proteins with known three-dimensional structures. The high-resolution structure of KD93 provides a first view of a member of the family of hypothetical proteins. And the structure provides a framework to deduce and assay the molecular function of other proteins of the UPF0113 family. |
==About this Structure== | ==About this Structure== | ||
| - | 1SQW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1SQW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: An, X | + | [[Category: An, X M.]] |
| - | [[Category: Chan, W | + | [[Category: Chan, W R.]] |
| - | [[Category: Chen, J | + | [[Category: Chen, J R.]] |
[[Category: Jiang, T.]] | [[Category: Jiang, T.]] | ||
| - | [[Category: Liang, D | + | [[Category: Liang, D C.]] |
| - | [[Category: Liu, J | + | [[Category: Liu, J F.]] |
| - | [[Category: Wang, X | + | [[Category: Wang, X Q.]] |
| - | [[Category: Wang, Z | + | [[Category: Wang, Z X.]] |
[[Category: pua]] | [[Category: pua]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:04:20 2008'' |
Revision as of 13:04, 21 February 2008
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Crystal structure of KD93, a novel protein expressed in the human pro
Overview
The crystal structure of a novel hypothetical protein, KD93, expressed in human hematopoietic stem/progenitor cells, was determined at 1.9A resolution using the multiple-wavelength anomalous dispersion (MAD) method. The protein KD93, which is encoded by the open reading frame HSPC031, is a NIP7 homologue and belongs to the UPF0113 family. The structural and functional information for the group of homologues has not yet been determined. Crystallographic analysis revealed that the overall fold of KD93 consists of two interlinked alpha/beta domains. Structure-based homology analysis with DALI revealed that the C domain of KD93 matches the PUA domain of some RNA modification enzymes, especially that of archaeosine tRNA-ribosyltransferase (ArcTGT), which suggests that its possible molecular function is related to RNA binding. The difference between the RNA binding regions of KD93 and ArcTGT in amino acid constitution and surface electrostatic potential indicate that they may have different RNA binding modes. The N domain of KD93 is a unique structure with no obvious similarity to other proteins with known three-dimensional structures. The high-resolution structure of KD93 provides a first view of a member of the family of hypothetical proteins. And the structure provides a framework to deduce and assay the molecular function of other proteins of the UPF0113 family.
About this Structure
1SQW is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of KD93, a novel protein expressed in human hematopoietic stem/progenitor cells., Liu JF, Wang XQ, Wang ZX, Chen JR, Jiang T, An XM, Chang WR, Liang DC, J Struct Biol. 2004 Dec;148(3):370-4. PMID:15522784
Page seeded by OCA on Thu Feb 21 15:04:20 2008
Categories: Homo sapiens | Single protein | An, X M. | Chan, W R. | Chen, J R. | Jiang, T. | Liang, D C. | Liu, J F. | Wang, X Q. | Wang, Z X. | Pua
