1ss6
From Proteopedia
(New page: 200px<br /> <applet load="1ss6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ss6" /> '''Solution structure of SEP domain from human...) |
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'''Solution structure of SEP domain from human p47'''<br /> | '''Solution structure of SEP domain from human p47'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The solution structure of the human p47 SEP domain in a construct | + | The solution structure of the human p47 SEP domain in a construct comprising residues G1-S2-p47(171-270) was determined by NMR spectroscopy. A structure-derived hypothesis about the domains' function was formulated and pursued in binding experiments with cysteine proteases. The SEP domain was found to be a reversible competitive inhibitor of cathepsin L with a Ki of 1.5 microM. The binding of G1-S2-p47(171-270) to cathepsin L was mapped by biochemical assays and the binding interface was investigated by NMR chemical shift perturbation experiments. |
==About this Structure== | ==About this Structure== | ||
| - | 1SS6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1SS6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SS6 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ball, L | + | [[Category: Ball, L J.]] |
[[Category: Buessow, K.]] | [[Category: Buessow, K.]] | ||
[[Category: Labudde, D.]] | [[Category: Labudde, D.]] | ||
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[[Category: nmr; p47; sep]] | [[Category: nmr; p47; sep]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:04:38 2008'' |
Revision as of 13:04, 21 February 2008
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Solution structure of SEP domain from human p47
Overview
The solution structure of the human p47 SEP domain in a construct comprising residues G1-S2-p47(171-270) was determined by NMR spectroscopy. A structure-derived hypothesis about the domains' function was formulated and pursued in binding experiments with cysteine proteases. The SEP domain was found to be a reversible competitive inhibitor of cathepsin L with a Ki of 1.5 microM. The binding of G1-S2-p47(171-270) to cathepsin L was mapped by biochemical assays and the binding interface was investigated by NMR chemical shift perturbation experiments.
About this Structure
1SS6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The SEP domain of p47 acts as a reversible competitive inhibitor of cathepsin L., Soukenik M, Diehl A, Leidert M, Sievert V, Bussow K, Leitner D, Labudde D, Ball LJ, Lechner A, Nagler DK, Oschkinat H, FEBS Lett. 2004 Oct 22;576(3):358-62. PMID:15498563
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