1st4
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Complete removal of residual N-7 guanine cap from degraded messenger RNA | + | Complete removal of residual N-7 guanine cap from degraded messenger RNA is necessary to prevent accumulation of intermediates that might interfere with RNA processing, export, and translation. The human scavenger decapping enzyme, DcpS, catalyzes residual cap hydrolysis following mRNA degradation, releasing N-7 methyl guanosine monophosphate and 5'-diphosphate terminated cap or mRNA products. DcpS structures bound to m(7)GpppG or m(7)GpppA reveal an asymmetric DcpS dimer that simultaneously creates an open nonproductive DcpS-cap complex and a closed productive DcpS-cap complex that alternate via 30 A domain movements. Structural and biochemical analysis suggests an autoregulatory mechanism whereby premature decapping mRNA is prevented by blocking the conformational changes that are required to form a closed productive active site capable of cap hydrolysis. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Gu, M.]] | [[Category: Gu, M.]] | ||
[[Category: Kiledjian, M.]] | [[Category: Kiledjian, M.]] | ||
| - | [[Category: Lima, C | + | [[Category: Lima, C D.]] |
[[Category: Liu, H.]] | [[Category: Liu, H.]] | ||
| - | [[Category: Liu, S | + | [[Category: Liu, S W.]] |
[[Category: GTA]] | [[Category: GTA]] | ||
[[Category: YT3]] | [[Category: YT3]] | ||
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[[Category: rna decay]] | [[Category: rna decay]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:04:54 2008'' |
Revision as of 13:04, 21 February 2008
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Structure of DcpS bound to m7GpppA
Overview
Complete removal of residual N-7 guanine cap from degraded messenger RNA is necessary to prevent accumulation of intermediates that might interfere with RNA processing, export, and translation. The human scavenger decapping enzyme, DcpS, catalyzes residual cap hydrolysis following mRNA degradation, releasing N-7 methyl guanosine monophosphate and 5'-diphosphate terminated cap or mRNA products. DcpS structures bound to m(7)GpppG or m(7)GpppA reveal an asymmetric DcpS dimer that simultaneously creates an open nonproductive DcpS-cap complex and a closed productive DcpS-cap complex that alternate via 30 A domain movements. Structural and biochemical analysis suggests an autoregulatory mechanism whereby premature decapping mRNA is prevented by blocking the conformational changes that are required to form a closed productive active site capable of cap hydrolysis.
About this Structure
1ST4 is a Single protein structure of sequence from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
Insights into the structure, mechanism, and regulation of scavenger mRNA decapping activity., Gu M, Fabrega C, Liu SW, Liu H, Kiledjian M, Lima CD, Mol Cell. 2004 Apr 9;14(1):67-80. PMID:15068804
Page seeded by OCA on Thu Feb 21 15:04:54 2008
Categories: Homo sapiens | Single protein | Fabrega, C. | Gu, M. | Kiledjian, M. | Lima, C D. | Liu, H. | Liu, S W. | GTA | YT3 | Cap | Decapping | Exosome | Hit protein | Messenger rna | Mrna | Rna decay
