1suu
From Proteopedia
(New page: 200px<br /><applet load="1suu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1suu, resolution 1.75Å" /> '''Structure of DNA gyr...) |
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| - | [[Image:1suu.gif|left|200px]]<br /><applet load="1suu" size=" | + | [[Image:1suu.gif|left|200px]]<br /><applet load="1suu" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1suu, resolution 1.75Å" /> | caption="1suu, resolution 1.75Å" /> | ||
'''Structure of DNA gyrase A C-terminal domain'''<br /> | '''Structure of DNA gyrase A C-terminal domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | DNA gyrase is unique among enzymes for its ability to actively introduce | + | DNA gyrase is unique among enzymes for its ability to actively introduce negative supercoils into DNA. This function is mediated in part by the C-terminal domain of its A subunit (GyrA CTD). Here, we report the crystal structure of this approximately 35-kDa domain determined to 1.75-A resolution. The GyrA CTD unexpectedly adopts an unusual fold, which we term a beta-pinwheel, that is globally reminiscent of a beta-propeller but is built of blades with a previously unobserved topology. A large, conserved basic patch on the outer edge of this domain suggests a likely site for binding and bending DNA; fluorescence resonance energy transfer-based assays show that the GyrA CTD is capable of bending DNA by > or =180 degrees over a 40-bp region. Surprisingly, we find that the CTD of the topoisomerase IV A subunit, which shares limited sequence homology with the GyrA CTD, also bends DNA. Together, these data provide a physical explanation for the ability of DNA gyrase to constrain a positive superhelical DNA wrap, and also suggest that the particular substrate preferences of topoisomerase IV might be dictated in part by the function of this domain. |
==About this Structure== | ==About this Structure== | ||
| - | 1SUU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi]. Active as [http://en.wikipedia.org/wiki/DNA_topoisomerase_(ATP-hydrolyzing) DNA topoisomerase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.3 5.99.1.3] Full crystallographic information is available from [http:// | + | 1SUU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Borrelia_burgdorferi Borrelia burgdorferi]. Active as [http://en.wikipedia.org/wiki/DNA_topoisomerase_(ATP-hydrolyzing) DNA topoisomerase (ATP-hydrolyzing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.3 5.99.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SUU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: DNA topoisomerase (ATP-hydrolyzing)]] | [[Category: DNA topoisomerase (ATP-hydrolyzing)]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Berger, J | + | [[Category: Berger, J M.]] |
| - | [[Category: Corbett, K | + | [[Category: Corbett, K D.]] |
| - | [[Category: Shultzaberger, R | + | [[Category: Shultzaberger, R K.]] |
[[Category: beta-pinwheel]] | [[Category: beta-pinwheel]] | ||
[[Category: beta-propeller]] | [[Category: beta-propeller]] | ||
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[[Category: topoisomerase]] | [[Category: topoisomerase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:05:25 2008'' |
Revision as of 13:05, 21 February 2008
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Structure of DNA gyrase A C-terminal domain
Overview
DNA gyrase is unique among enzymes for its ability to actively introduce negative supercoils into DNA. This function is mediated in part by the C-terminal domain of its A subunit (GyrA CTD). Here, we report the crystal structure of this approximately 35-kDa domain determined to 1.75-A resolution. The GyrA CTD unexpectedly adopts an unusual fold, which we term a beta-pinwheel, that is globally reminiscent of a beta-propeller but is built of blades with a previously unobserved topology. A large, conserved basic patch on the outer edge of this domain suggests a likely site for binding and bending DNA; fluorescence resonance energy transfer-based assays show that the GyrA CTD is capable of bending DNA by > or =180 degrees over a 40-bp region. Surprisingly, we find that the CTD of the topoisomerase IV A subunit, which shares limited sequence homology with the GyrA CTD, also bends DNA. Together, these data provide a physical explanation for the ability of DNA gyrase to constrain a positive superhelical DNA wrap, and also suggest that the particular substrate preferences of topoisomerase IV might be dictated in part by the function of this domain.
About this Structure
1SUU is a Single protein structure of sequence from Borrelia burgdorferi. Active as DNA topoisomerase (ATP-hydrolyzing), with EC number 5.99.1.3 Full crystallographic information is available from OCA.
Reference
The C-terminal domain of DNA gyrase A adopts a DNA-bending beta-pinwheel fold., Corbett KD, Shultzaberger RK, Berger JM, Proc Natl Acad Sci U S A. 2004 May 11;101(19):7293-8. Epub 2004 May 3. PMID:15123801
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