1svf
From Proteopedia
(New page: 200px<br /><applet load="1svf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1svf, resolution 1.4Å" /> '''PARAMYXOVIRUS SV5 FUS...) |
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| - | [[Image:1svf.jpg|left|200px]]<br /><applet load="1svf" size=" | + | [[Image:1svf.jpg|left|200px]]<br /><applet load="1svf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1svf, resolution 1.4Å" /> | caption="1svf, resolution 1.4Å" /> | ||
'''PARAMYXOVIRUS SV5 FUSION PROTEIN CORE'''<br /> | '''PARAMYXOVIRUS SV5 FUSION PROTEIN CORE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Paramyxoviruses are responsible for significant human mortality and | + | Paramyxoviruses are responsible for significant human mortality and disease worldwide, but the molecular mechanisms underlying their entry into host cells remain poorly understood. We have solved the crystal structure of a fragment of the simian parainfluenza virus 5 fusion protein (SV5 F), revealing a 96 A long coiled coil surrounded by three antiparallel helices. This structure places the fusion and transmembrane anchor of SV5 F in close proximity with a large intervening domain at the opposite end of the coiled coil. Six amino acids, potentially part of the fusion peptide, form a segment of the central coiled coil, suggesting that this structure extends into the membrane. Deletion mutants of SV5 F indicate that putative flexible tethers between the coiled coil and the viral membrane are dispensable for fusion. The lack of flexible tethers may couple a final conformational change in the F protein directly to the fusion of two bilayers. |
==About this Structure== | ==About this Structure== | ||
| - | 1SVF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1SVF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SVF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Simian virus 40]] | [[Category: Simian virus 40]] | ||
| - | [[Category: Baker, K | + | [[Category: Baker, K A.]] |
| - | [[Category: Dutch, R | + | [[Category: Dutch, R E.]] |
| - | [[Category: Jardetzky, T | + | [[Category: Jardetzky, T S.]] |
| - | [[Category: Lamb, R | + | [[Category: Lamb, R A.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: coiled-coil]] | [[Category: coiled-coil]] | ||
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[[Category: sv5]] | [[Category: sv5]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:05:39 2008'' |
Revision as of 13:05, 21 February 2008
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PARAMYXOVIRUS SV5 FUSION PROTEIN CORE
Overview
Paramyxoviruses are responsible for significant human mortality and disease worldwide, but the molecular mechanisms underlying their entry into host cells remain poorly understood. We have solved the crystal structure of a fragment of the simian parainfluenza virus 5 fusion protein (SV5 F), revealing a 96 A long coiled coil surrounded by three antiparallel helices. This structure places the fusion and transmembrane anchor of SV5 F in close proximity with a large intervening domain at the opposite end of the coiled coil. Six amino acids, potentially part of the fusion peptide, form a segment of the central coiled coil, suggesting that this structure extends into the membrane. Deletion mutants of SV5 F indicate that putative flexible tethers between the coiled coil and the viral membrane are dispensable for fusion. The lack of flexible tethers may couple a final conformational change in the F protein directly to the fusion of two bilayers.
About this Structure
1SVF is a Protein complex structure of sequences from Simian virus 40 with as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis for paramyxovirus-mediated membrane fusion., Baker KA, Dutch RE, Lamb RA, Jardetzky TS, Mol Cell. 1999 Mar;3(3):309-19. PMID:10198633
Page seeded by OCA on Thu Feb 21 15:05:39 2008
Categories: Protein complex | Simian virus 40 | Baker, K A. | Dutch, R E. | Jardetzky, T S. | Lamb, R A. | CL | Coiled-coil | Fusion | Paramyxovirus | Sv5
