Cephalosporin acylase
From Proteopedia
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Michal Harel (Talk | contribs)
(New page: '''Cephalosporin acylase''' (CSA) catalyzes the conversion of cephalosporin C (CPC) to 7-amino-cephalosporanic acid (7-ACA). CSA contains 2 non-identical subunits. CSA is used commerci...)
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Revision as of 10:57, 26 December 2012
Cephalosporin acylase (CSA) catalyzes the conversion of cephalosporin C (CPC) to 7-amino-cephalosporanic acid (7-ACA). CSA contains 2 non-identical subunits. CSA is used commercially for production of semi-synthetic cephalosporin antibiotics. The active site pocket of CSA is similar to that of penicillin acylase. CSA precursor is cleaved at 2 sites forming the active heterodimer.
3D structures of cephalosporin acylase
2nlz - CSA – Bacillus halodurans
1fm2 - BdCSA – Brevundimonas diminuta
1gk0, 1gk1, 1ghd - PsCSA – Pseudomonas
2ae3, 2ae5 - PsCSA α + β (mutant) subunits
2ae4 - PsCSA α (mutant) + β subunits
1keh - BdCSA precursor (mutant)
