1szp
From Proteopedia
(New page: 200px<br /><applet load="1szp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1szp, resolution 3.25Å" /> '''A Crystal Structure ...) |
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| - | [[Image:1szp.gif|left|200px]]<br /><applet load="1szp" size=" | + | [[Image:1szp.gif|left|200px]]<br /><applet load="1szp" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1szp, resolution 3.25Å" /> | caption="1szp, resolution 3.25Å" /> | ||
'''A Crystal Structure of the Rad51 Filament'''<br /> | '''A Crystal Structure of the Rad51 Filament'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Rad51, the major eukaryotic homologous recombinase, is important for the | + | Rad51, the major eukaryotic homologous recombinase, is important for the repair of DNA damage and the maintenance of genomic diversity and stability. The active form of this DNA-dependent ATPase is a helical filament within which the search for homology and strand exchange occurs. Here we present the crystal structure of a Saccharomyces cerevisiae Rad51 filament formed by a gain-of-function mutant. This filament has a longer pitch than that seen in crystals of Rad51's prokaryotic homolog RecA, and places the ATPase site directly at a new interface between protomers. Although the filament exhibits approximate six-fold symmetry, alternate protein-protein interfaces are slightly different, implying that the functional unit of Rad51 within the filament may be a dimer. Additionally, we show that mutation of His352, which lies at this new interface, markedly disrupts DNA binding. |
==About this Structure== | ==About this Structure== | ||
| - | 1SZP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1SZP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Conway, A | + | [[Category: Conway, A B.]] |
| - | [[Category: Fortin, G | + | [[Category: Fortin, G S.]] |
| - | [[Category: Lynch, T | + | [[Category: Lynch, T W.]] |
| - | [[Category: Rice, P | + | [[Category: Rice, P A.]] |
| - | [[Category: Symington, L | + | [[Category: Symington, L S.]] |
[[Category: Zhang, Y.]] | [[Category: Zhang, Y.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: rad51 filament]] | [[Category: rad51 filament]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:08:01 2008'' |
Revision as of 13:08, 21 February 2008
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A Crystal Structure of the Rad51 Filament
Overview
Rad51, the major eukaryotic homologous recombinase, is important for the repair of DNA damage and the maintenance of genomic diversity and stability. The active form of this DNA-dependent ATPase is a helical filament within which the search for homology and strand exchange occurs. Here we present the crystal structure of a Saccharomyces cerevisiae Rad51 filament formed by a gain-of-function mutant. This filament has a longer pitch than that seen in crystals of Rad51's prokaryotic homolog RecA, and places the ATPase site directly at a new interface between protomers. Although the filament exhibits approximate six-fold symmetry, alternate protein-protein interfaces are slightly different, implying that the functional unit of Rad51 within the filament may be a dimer. Additionally, we show that mutation of His352, which lies at this new interface, markedly disrupts DNA binding.
About this Structure
1SZP is a Single protein structure of sequence from Saccharomyces cerevisiae with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a Rad51 filament., Conway AB, Lynch TW, Zhang Y, Fortin GS, Fung CW, Symington LS, Rice PA, Nat Struct Mol Biol. 2004 Aug;11(8):791-6. Epub 2004 Jul 4. PMID:15235592
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