1t01
From Proteopedia
(New page: 200px<br /><applet load="1t01" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t01, resolution 2.06Å" /> '''Vinculin complexed w...) |
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| - | [[Image:1t01.gif|left|200px]]<br /><applet load="1t01" size=" | + | [[Image:1t01.gif|left|200px]]<br /><applet load="1t01" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1t01, resolution 2.06Å" /> | caption="1t01, resolution 2.06Å" /> | ||
'''Vinculin complexed with the VBS1 helix from talin'''<br /> | '''Vinculin complexed with the VBS1 helix from talin'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The interaction between the cytoskeletal proteins talin and vinculin plays | + | The interaction between the cytoskeletal proteins talin and vinculin plays a key role in integrin-mediated cell adhesion and migration. We have determined the crystal structures of two domains from the talin rod spanning residues 482-789. Talin 482-655, which contains a vinculin-binding site (VBS), folds into a five-helix bundle whereas talin 656-789 is a four-helix bundle. We show that the VBS is composed of a hydrophobic surface spanning five turns of helix 4. All the key side chains from the VBS are buried and contribute to the hydrophobic core of the talin 482-655 fold. We demonstrate that the talin 482-655 five-helix bundle represents an inactive conformation, and mutations that disrupt the hydrophobic core or deletion of helix 5 are required to induce an active conformation in which the VBS is exposed. We also report the crystal structure of the N-terminal vinculin head domain in complex with an activated form of talin. Activation of the VBS in talin and the recruitment of vinculin may support the maturation of small integrin/talin complexes into more stable adhesions. |
==About this Structure== | ==About this Structure== | ||
| - | 1T01 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http:// | + | 1T01 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T01 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Barsukov, I | + | [[Category: Barsukov, I L.]] |
| - | [[Category: Critchley, D | + | [[Category: Critchley, D R.]] |
[[Category: Emsley, J.]] | [[Category: Emsley, J.]] | ||
| - | [[Category: Gingras, A | + | [[Category: Gingras, A R.]] |
[[Category: Papagrigoriou, E.]] | [[Category: Papagrigoriou, E.]] | ||
[[Category: five helix bundle]] | [[Category: five helix bundle]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:08:12 2008'' |
Revision as of 13:08, 21 February 2008
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Vinculin complexed with the VBS1 helix from talin
Overview
The interaction between the cytoskeletal proteins talin and vinculin plays a key role in integrin-mediated cell adhesion and migration. We have determined the crystal structures of two domains from the talin rod spanning residues 482-789. Talin 482-655, which contains a vinculin-binding site (VBS), folds into a five-helix bundle whereas talin 656-789 is a four-helix bundle. We show that the VBS is composed of a hydrophobic surface spanning five turns of helix 4. All the key side chains from the VBS are buried and contribute to the hydrophobic core of the talin 482-655 fold. We demonstrate that the talin 482-655 five-helix bundle represents an inactive conformation, and mutations that disrupt the hydrophobic core or deletion of helix 5 are required to induce an active conformation in which the VBS is exposed. We also report the crystal structure of the N-terminal vinculin head domain in complex with an activated form of talin. Activation of the VBS in talin and the recruitment of vinculin may support the maturation of small integrin/talin complexes into more stable adhesions.
About this Structure
1T01 is a Protein complex structure of sequences from Gallus gallus and Mus musculus. Full crystallographic information is available from OCA.
Reference
Activation of a vinculin-binding site in the talin rod involves rearrangement of a five-helix bundle., Papagrigoriou E, Gingras AR, Barsukov IL, Bate N, Fillingham IJ, Patel B, Frank R, Ziegler WH, Roberts GC, Critchley DR, Emsley J, EMBO J. 2004 Aug 4;23(15):2942-51. Epub 2004 Jul 22. PMID:15272303
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