1szw
From Proteopedia
(New page: 200px<br /><applet load="1szw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1szw, resolution 2.00Å" /> '''Crystal structure of...) |
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| - | [[Image:1szw.gif|left|200px]]<br /><applet load="1szw" size=" | + | [[Image:1szw.gif|left|200px]]<br /><applet load="1szw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1szw, resolution 2.00Å" /> | caption="1szw, resolution 2.00Å" /> | ||
'''Crystal structure of E. coli tRNA pseudouridine synthase TruD'''<br /> | '''Crystal structure of E. coli tRNA pseudouridine synthase TruD'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Pseudouridine synthases catalyse the isomerisation of uridine to | + | Pseudouridine synthases catalyse the isomerisation of uridine to pseudouridine in structural RNA. The pseudouridine synthase TruD, that modifies U13 in tRNA, belongs to a recently identified and large family of pseudouridine synthases present in all kingdoms of life. We report here the crystal structure of Escherichia coli TruD at 2.0 A resolution. The structure reveals an overall V-shaped molecule with an RNA-binding cleft formed between two domains: a catalytic domain and an insertion domain. The catalytic domain has a fold similar to that of the catalytic domains of previously characterised pseudouridine synthases, whereas the insertion domain displays a novel fold. |
==About this Structure== | ==About this Structure== | ||
| - | 1SZW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] Full crystallographic information is available from [http:// | + | 1SZW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Active as [http://en.wikipedia.org/wiki/Pseudouridylate_synthase Pseudouridylate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.70 4.2.1.70] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pseudouridylate synthase]] | [[Category: Pseudouridylate synthase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ericsson, U | + | [[Category: Ericsson, U B.]] |
| - | [[Category: Hallberg, B | + | [[Category: Hallberg, B M.]] |
[[Category: Nordlund, P.]] | [[Category: Nordlund, P.]] | ||
| - | [[Category: SPINE, Structural | + | [[Category: SPINE, Structural Proteomics in Europe.]] |
[[Category: novel fold]] | [[Category: novel fold]] | ||
[[Category: pseudouridine synthase]] | [[Category: pseudouridine synthase]] | ||
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[[Category: trud]] | [[Category: trud]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:08:07 2008'' |
Revision as of 13:08, 21 February 2008
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Crystal structure of E. coli tRNA pseudouridine synthase TruD
Overview
Pseudouridine synthases catalyse the isomerisation of uridine to pseudouridine in structural RNA. The pseudouridine synthase TruD, that modifies U13 in tRNA, belongs to a recently identified and large family of pseudouridine synthases present in all kingdoms of life. We report here the crystal structure of Escherichia coli TruD at 2.0 A resolution. The structure reveals an overall V-shaped molecule with an RNA-binding cleft formed between two domains: a catalytic domain and an insertion domain. The catalytic domain has a fold similar to that of the catalytic domains of previously characterised pseudouridine synthases, whereas the insertion domain displays a novel fold.
About this Structure
1SZW is a Single protein structure of sequence from Escherichia coli. Active as Pseudouridylate synthase, with EC number 4.2.1.70 Full crystallographic information is available from OCA.
Reference
X-ray structure of tRNA pseudouridine synthase TruD reveals an inserted domain with a novel fold., Ericsson UB, Nordlund P, Hallberg BM, FEBS Lett. 2004 May 7;565(1-3):59-64. PMID:15135053
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