1t0p
From Proteopedia
(New page: 200px<br /> <applet load="1t0p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t0p, resolution 1.66Å" /> '''Structural Basis of...) |
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| - | [[Image:1t0p.gif|left|200px]]<br /> | + | [[Image:1t0p.gif|left|200px]]<br /><applet load="1t0p" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1t0p" size=" | + | |
caption="1t0p, resolution 1.66Å" /> | caption="1t0p, resolution 1.66Å" /> | ||
'''Structural Basis of ICAM recognition by integrin alpahLbeta2 revealed in the complex structure of binding domains of ICAM-3 and alphaLbeta2 at 1.65 A'''<br /> | '''Structural Basis of ICAM recognition by integrin alpahLbeta2 revealed in the complex structure of binding domains of ICAM-3 and alphaLbeta2 at 1.65 A'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Within the Ig superfamily (IgSF), intercellular adhesion molecules (ICAMs) | + | Within the Ig superfamily (IgSF), intercellular adhesion molecules (ICAMs) form a subfamily that binds the leukocyte integrin alphaLbeta2. We report a 1.65-A-resolution crystal structure of the ICAM-3 N-terminal domain (D1) in complex with the inserted domain, the ligand-binding domain of alphaLbeta2. This high-resolution structure and comparisons among ICAM subfamily members establish that the binding of ICAM-3 D1 onto the inserted domain represents a common docking mode for ICAM subfamily members. The markedly different off-rates of ICAM-1, -2, and -3 appear to be determined by the hydrophobicity of residues that surround a metal coordination bond in the alphaLbeta2-binding interfaces. Variation in composition of glycans on the periphery of the interfaces influences on-rate. |
==About this Structure== | ==About this Structure== | ||
| - | 1T0P is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NAG and MG as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1T0P is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=NAG:'>NAG</scene> and <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0P OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Liu, J | + | [[Category: Liu, J H.]] |
[[Category: Shimaoko, M.]] | [[Category: Shimaoko, M.]] | ||
[[Category: Song, G.]] | [[Category: Song, G.]] | ||
| - | [[Category: Springer, T | + | [[Category: Springer, T A.]] |
| - | [[Category: Wang, J | + | [[Category: Wang, J H.]] |
| - | [[Category: Yang, Y | + | [[Category: Yang, Y T.]] |
[[Category: MG]] | [[Category: MG]] | ||
[[Category: NAG]] | [[Category: NAG]] | ||
[[Category: rossmann fold; ig-super family domain]] | [[Category: rossmann fold; ig-super family domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:08:34 2008'' |
Revision as of 13:08, 21 February 2008
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Structural Basis of ICAM recognition by integrin alpahLbeta2 revealed in the complex structure of binding domains of ICAM-3 and alphaLbeta2 at 1.65 A
Overview
Within the Ig superfamily (IgSF), intercellular adhesion molecules (ICAMs) form a subfamily that binds the leukocyte integrin alphaLbeta2. We report a 1.65-A-resolution crystal structure of the ICAM-3 N-terminal domain (D1) in complex with the inserted domain, the ligand-binding domain of alphaLbeta2. This high-resolution structure and comparisons among ICAM subfamily members establish that the binding of ICAM-3 D1 onto the inserted domain represents a common docking mode for ICAM subfamily members. The markedly different off-rates of ICAM-1, -2, and -3 appear to be determined by the hydrophobicity of residues that surround a metal coordination bond in the alphaLbeta2-binding interfaces. Variation in composition of glycans on the periphery of the interfaces influences on-rate.
About this Structure
1T0P is a Protein complex structure of sequences from Homo sapiens with and as ligands. Full crystallographic information is available from OCA.
Reference
An atomic resolution view of ICAM recognition in a complex between the binding domains of ICAM-3 and integrin alphaLbeta2., Song G, Yang Y, Liu JH, Casasnovas JM, Shimaoka M, Springer TA, Wang JH, Proc Natl Acad Sci U S A. 2005 Mar 1;102(9):3366-71. Epub 2005 Feb 22. PMID:15728350
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