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1t1d
From Proteopedia
(New page: 200px<br /><applet load="1t1d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t1d, resolution 1.51Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1t1d.gif|left|200px]]<br /><applet load="1t1d" size=" | + | [[Image:1t1d.gif|left|200px]]<br /><applet load="1t1d" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1t1d, resolution 1.51Å" /> | caption="1t1d, resolution 1.51Å" /> | ||
'''CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POTASSIUM CHANNEL'''<br /> | '''CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POTASSIUM CHANNEL'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The N-terminal, cytoplasmic tetramerization domain (T1) of voltage-gated | + | The N-terminal, cytoplasmic tetramerization domain (T1) of voltage-gated K+ channels encodes molecular determinants for subfamily-specific assembly of alpha-subunits into functional tetrameric channels. Crystal structures of T1 tetramers from Shaw and Shaker subfamilies reveal a common four-layered scaffolding. Within layer 4, on the hypothetical membrane-facing side of the tetramer, the Shaw T1 tetramer contains four zinc ions; each is coordinated by a histidine and two cysteines from one monomer and by one cysteine from an adjacent monomer. The amino acids involved in coordinating the Zn2+ ion occur in a HX5CX20CC sequence motif that is highly conserved among all Shab, Shaw and Shal subfamily members, but is not found in Shaker subfamily members. We demonstrate by coimmunoprecipitation that a few characteristic residues in the subunit interface are crucial for subfamily-specific tetramerization of the T1 domains. |
==About this Structure== | ==About this Structure== | ||
| - | 1T1D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http:// | + | 1T1D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aplysia_californica Aplysia californica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1D OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Choe, S.]] | [[Category: Choe, S.]] | ||
[[Category: Kreusch, A.]] | [[Category: Kreusch, A.]] | ||
| - | [[Category: Pfaffinger, P | + | [[Category: Pfaffinger, P J.]] |
| - | [[Category: Stevens, C | + | [[Category: Stevens, C F.]] |
| - | [[Category: aplysia kv1 | + | [[Category: aplysia kv1 1]] |
[[Category: potassium channels]] | [[Category: potassium channels]] | ||
[[Category: proton transport]] | [[Category: proton transport]] | ||
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[[Category: x-ray structure]] | [[Category: x-ray structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:08:53 2008'' |
Revision as of 13:08, 21 February 2008
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CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POTASSIUM CHANNEL
Overview
The N-terminal, cytoplasmic tetramerization domain (T1) of voltage-gated K+ channels encodes molecular determinants for subfamily-specific assembly of alpha-subunits into functional tetrameric channels. Crystal structures of T1 tetramers from Shaw and Shaker subfamilies reveal a common four-layered scaffolding. Within layer 4, on the hypothetical membrane-facing side of the tetramer, the Shaw T1 tetramer contains four zinc ions; each is coordinated by a histidine and two cysteines from one monomer and by one cysteine from an adjacent monomer. The amino acids involved in coordinating the Zn2+ ion occur in a HX5CX20CC sequence motif that is highly conserved among all Shab, Shaw and Shal subfamily members, but is not found in Shaker subfamily members. We demonstrate by coimmunoprecipitation that a few characteristic residues in the subunit interface are crucial for subfamily-specific tetramerization of the T1 domains.
About this Structure
1T1D is a Single protein structure of sequence from Aplysia californica. Full crystallographic information is available from OCA.
Reference
Zn2+-binding and molecular determinants of tetramerization in voltage-gated K+ channels., Bixby KA, Nanao MH, Shen NV, Kreusch A, Bellamy H, Pfaffinger PJ, Choe S, Nat Struct Biol. 1999 Jan;6(1):38-43. PMID:9886290
Page seeded by OCA on Thu Feb 21 15:08:53 2008
