1t2r
From Proteopedia
(New page: 200px<br /><applet load="1t2r" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t2r" /> '''Structural basis for 3' end recognition of n...) |
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| - | [[Image:1t2r.gif|left|200px]]<br /><applet load="1t2r" size=" | + | [[Image:1t2r.gif|left|200px]]<br /><applet load="1t2r" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1t2r" /> | caption="1t2r" /> | ||
'''Structural basis for 3' end recognition of nucleic acids by the Drosophila Argonaute 2 PAZ domain'''<br /> | '''Structural basis for 3' end recognition of nucleic acids by the Drosophila Argonaute 2 PAZ domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We describe the solution structures of the Argonaute2 PAZ domain bound to | + | We describe the solution structures of the Argonaute2 PAZ domain bound to RNA and DNA oligonucleotides. The structures reveal a unique mode of single-stranded nucleic acid binding in which the two 3'-terminal nucleotides are buried in a hydrophobic cleft. We propose that the PAZ domain contributes to the specific recognition of siRNAs by providing a binding pocket for their characteristic two-nucleotide 3' overhangs. |
==About this Structure== | ==About this Structure== | ||
| - | 1T2R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http:// | + | 1T2R is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2R OCA]. |
==Reference== | ==Reference== | ||
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[[Category: rna]] | [[Category: rna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:09:15 2008'' |
Revision as of 13:09, 21 February 2008
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Structural basis for 3' end recognition of nucleic acids by the Drosophila Argonaute 2 PAZ domain
Overview
We describe the solution structures of the Argonaute2 PAZ domain bound to RNA and DNA oligonucleotides. The structures reveal a unique mode of single-stranded nucleic acid binding in which the two 3'-terminal nucleotides are buried in a hydrophobic cleft. We propose that the PAZ domain contributes to the specific recognition of siRNAs by providing a binding pocket for their characteristic two-nucleotide 3' overhangs.
About this Structure
1T2R is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Nucleic acid 3'-end recognition by the Argonaute2 PAZ domain., Lingel A, Simon B, Izaurralde E, Sattler M, Nat Struct Mol Biol. 2004 Jun;11(6):576-7. Epub 2004 May 23. PMID:15156196
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