1t3b
From Proteopedia
(New page: 200px<br /><applet load="1t3b" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t3b, resolution 2.5Å" /> '''X-ray Structure of Ds...) |
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| - | [[Image:1t3b.gif|left|200px]]<br /><applet load="1t3b" size=" | + | [[Image:1t3b.gif|left|200px]]<br /><applet load="1t3b" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1t3b, resolution 2.5Å" /> | caption="1t3b, resolution 2.5Å" /> | ||
'''X-ray Structure of DsbC from Haemophilus influenzae'''<br /> | '''X-ray Structure of DsbC from Haemophilus influenzae'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Bacterial DsbC proteins are involved in rearranging or reducing mismatched | + | Bacterial DsbC proteins are involved in rearranging or reducing mismatched disulfide bonds folding within the periplasm. The X-ray structure of the enzyme from Haemophilus influenzae has been solved and compared with the known structure of the Escherichia coli protein. The proteins act as V-shaped dimers with a large cleft to accommodate substrate proteins. The dimers are anchored by a small N-terminal domain, but have a flexible linker region which allows the larger C-terminal domain, with its reactive sulfhydryls, to clamp down on substrates. The overall folds are very similar, but the comparison shows a wider range of hinge motions than previously thought. The crystal packing of the H. influenzae protein allows the movement of the N-terminal domain with respect to the C-terminal domain through motions in the flexible hinge, generating high thermal parameters and unusually high anisotropy in the crystallographic data. |
==About this Structure== | ==About this Structure== | ||
| - | 1T3B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Active as [http://en.wikipedia.org/wiki/Protein_disulfide-isomerase Protein disulfide-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.4.1 5.3.4.1] Full crystallographic information is available from [http:// | + | 1T3B is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Active as [http://en.wikipedia.org/wiki/Protein_disulfide-isomerase Protein disulfide-isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.4.1 5.3.4.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T3B OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Georgiou, G.]] | [[Category: Georgiou, G.]] | ||
| - | [[Category: Monzingo, A | + | [[Category: Monzingo, A F.]] |
| - | [[Category: Robertus, J | + | [[Category: Robertus, J D.]] |
[[Category: Segatori, L.]] | [[Category: Segatori, L.]] | ||
[[Category: Zhang, M.]] | [[Category: Zhang, M.]] | ||
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[[Category: redox-active center]] | [[Category: redox-active center]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:09:27 2008'' |
Revision as of 13:09, 21 February 2008
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X-ray Structure of DsbC from Haemophilus influenzae
Overview
Bacterial DsbC proteins are involved in rearranging or reducing mismatched disulfide bonds folding within the periplasm. The X-ray structure of the enzyme from Haemophilus influenzae has been solved and compared with the known structure of the Escherichia coli protein. The proteins act as V-shaped dimers with a large cleft to accommodate substrate proteins. The dimers are anchored by a small N-terminal domain, but have a flexible linker region which allows the larger C-terminal domain, with its reactive sulfhydryls, to clamp down on substrates. The overall folds are very similar, but the comparison shows a wider range of hinge motions than previously thought. The crystal packing of the H. influenzae protein allows the movement of the N-terminal domain with respect to the C-terminal domain through motions in the flexible hinge, generating high thermal parameters and unusually high anisotropy in the crystallographic data.
About this Structure
1T3B is a Single protein structure of sequence from Haemophilus influenzae. Active as Protein disulfide-isomerase, with EC number 5.3.4.1 Full crystallographic information is available from OCA.
Reference
Structure of DsbC from Haemophilus influenzae., Zhang M, Monzingo AF, Segatori L, Georgiou G, Robertus JD, Acta Crystallogr D Biol Crystallogr. 2004 Sep;60(Pt 9):1512-8. Epub 2004, Aug 26. PMID:15333920
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