1t5c
From Proteopedia
(New page: 200px<br /> <applet load="1t5c" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t5c, resolution 2.5Å" /> '''Crystal structure of...) |
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| - | [[Image:1t5c.gif|left|200px]]<br /> | + | [[Image:1t5c.gif|left|200px]]<br /><applet load="1t5c" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="1t5c, resolution 2.5Å" /> | caption="1t5c, resolution 2.5Å" /> | ||
'''Crystal structure of the motor domain of human kinetochore protein CENP-E'''<br /> | '''Crystal structure of the motor domain of human kinetochore protein CENP-E'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The human kinetochore is a highly complex macromolecular structure that | + | The human kinetochore is a highly complex macromolecular structure that connects chromosomes to spindle microtubules (MTs) in order to facilitate accurate chromosome segregation. Centromere-associated protein E (CENP-E), a member of the kinesin superfamily, is an essential component of the kinetochore, since it is required to stabilize the attachment of chromosomes to spindle MTs, to develop tension across aligned chromosomes, to stabilize spindle poles and to satisfy the mitotic checkpoint. Here we report the 2.5A resolution crystal structure of the motor domain and linker region of human CENP-E with MgADP bound in the active site. This structure displays subtle but important differences compared to the structures of human Eg5 and conventional kinesin. Our structure reveals that the CENP-E linker region is in a "docked" position identical to that in the human plus-end directed conventional kinesin. CENP-E has many advantages as a potential anti-mitotic drug target and this crystal structure of human CENP-E will provide a starting point for high throughput virtual screening of potential inhibitors. |
==About this Structure== | ==About this Structure== | ||
| - | 1T5C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with MG, NO3, ADP and PIN as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1T5C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=MG:'>MG</scene>, <scene name='pdbligand=NO3:'>NO3</scene>, <scene name='pdbligand=ADP:'>ADP</scene> and <scene name='pdbligand=PIN:'>PIN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T5C OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Garcia-Saez, I.]] | [[Category: Garcia-Saez, I.]] | ||
[[Category: Kozielski, F.]] | [[Category: Kozielski, F.]] | ||
| - | [[Category: SPINE, Structural | + | [[Category: SPINE, Structural Proteomics in Europe.]] |
| - | [[Category: Wade, R | + | [[Category: Wade, R H.]] |
[[Category: Yen, T.]] | [[Category: Yen, T.]] | ||
[[Category: ADP]] | [[Category: ADP]] | ||
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[[Category: structural proteomics in europe]] | [[Category: structural proteomics in europe]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:10:01 2008'' |
Revision as of 13:10, 21 February 2008
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Crystal structure of the motor domain of human kinetochore protein CENP-E
Overview
The human kinetochore is a highly complex macromolecular structure that connects chromosomes to spindle microtubules (MTs) in order to facilitate accurate chromosome segregation. Centromere-associated protein E (CENP-E), a member of the kinesin superfamily, is an essential component of the kinetochore, since it is required to stabilize the attachment of chromosomes to spindle MTs, to develop tension across aligned chromosomes, to stabilize spindle poles and to satisfy the mitotic checkpoint. Here we report the 2.5A resolution crystal structure of the motor domain and linker region of human CENP-E with MgADP bound in the active site. This structure displays subtle but important differences compared to the structures of human Eg5 and conventional kinesin. Our structure reveals that the CENP-E linker region is in a "docked" position identical to that in the human plus-end directed conventional kinesin. CENP-E has many advantages as a potential anti-mitotic drug target and this crystal structure of human CENP-E will provide a starting point for high throughput virtual screening of potential inhibitors.
About this Structure
1T5C is a Single protein structure of sequence from Homo sapiens with , , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of the motor domain of the human kinetochore protein CENP-E., Garcia-Saez I, Yen T, Wade RH, Kozielski F, J Mol Biol. 2004 Jul 23;340(5):1107-16. PMID:15236970
Page seeded by OCA on Thu Feb 21 15:10:01 2008
Categories: Homo sapiens | Single protein | Garcia-Saez, I. | Kozielski, F. | SPINE, Structural Proteomics in Europe. | Wade, R H. | Yen, T. | ADP | MG | NO3 | PIN | Arrow-head shape | Kinesin motor-domain-adp complex | Spine | Stranded beta-sheet core with solvent exposed alpha-helices | Structural genomics | Structural proteomics in europe
