1t6v
From Proteopedia
(New page: 200px<br /> <applet load="1t6v" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t6v, resolution 1.70Å" /> '''Crystal structure a...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1t6v.gif|left|200px]]<br /> | + | [[Image:1t6v.gif|left|200px]]<br /><applet load="1t6v" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1t6v" size=" | + | |
caption="1t6v, resolution 1.70Å" /> | caption="1t6v, resolution 1.70Å" /> | ||
'''Crystal structure analysis of the nurse shark new antigen receptor (NAR) variable domain in complex with lysozyme'''<br /> | '''Crystal structure analysis of the nurse shark new antigen receptor (NAR) variable domain in complex with lysozyme'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cartilaginous fish are the phylogenetically oldest living organisms known | + | Cartilaginous fish are the phylogenetically oldest living organisms known to possess components of the vertebrate adaptive immune system. Key to their immune response are heavy-chain, homodimeric immunoglobulins called new antigen receptors (IgNARs), in which the variable (V) domains recognize antigens with only a single immunoglobulin domain, akin to camelid heavy-chain V domains. The 1.45 angstrom resolution crystal structure of the type I IgNAR V domain in complex with hen egg-white lysozyme (HEL) reveals a minimal antigen-binding domain that contains only two of the three conventional complementarity-determining regions but still binds HEL with nanomolar affinity by means of a binding interface comparable in size to conventional antibodies. |
==About this Structure== | ==About this Structure== | ||
| - | 1T6V is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Ginglymostoma_cirratum Ginglymostoma cirratum] with CL as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http:// | + | 1T6V is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [http://en.wikipedia.org/wiki/Ginglymostoma_cirratum Ginglymostoma cirratum] with <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T6V OCA]. |
==Reference== | ==Reference== | ||
| Line 17: | Line 16: | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Dooley, H.]] | [[Category: Dooley, H.]] | ||
| - | [[Category: Flajnik, M | + | [[Category: Flajnik, M F.]] |
| - | [[Category: Stanfield, R | + | [[Category: Stanfield, R L.]] |
| - | [[Category: Wilson, I | + | [[Category: Wilson, I A.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: immunoglobulin fold]] | [[Category: immunoglobulin fold]] | ||
[[Category: protein-protein complex]] | [[Category: protein-protein complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:10:29 2008'' |
Revision as of 13:10, 21 February 2008
|
Crystal structure analysis of the nurse shark new antigen receptor (NAR) variable domain in complex with lysozyme
Overview
Cartilaginous fish are the phylogenetically oldest living organisms known to possess components of the vertebrate adaptive immune system. Key to their immune response are heavy-chain, homodimeric immunoglobulins called new antigen receptors (IgNARs), in which the variable (V) domains recognize antigens with only a single immunoglobulin domain, akin to camelid heavy-chain V domains. The 1.45 angstrom resolution crystal structure of the type I IgNAR V domain in complex with hen egg-white lysozyme (HEL) reveals a minimal antigen-binding domain that contains only two of the three conventional complementarity-determining regions but still binds HEL with nanomolar affinity by means of a binding interface comparable in size to conventional antibodies.
About this Structure
1T6V is a Protein complex structure of sequences from Gallus gallus and Ginglymostoma cirratum with as ligand. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
Crystal structure of a shark single-domain antibody V region in complex with lysozyme., Stanfield RL, Dooley H, Flajnik MF, Wilson IA, Science. 2004 Sep 17;305(5691):1770-3. Epub 2004 Aug 19. PMID:15319492
Page seeded by OCA on Thu Feb 21 15:10:29 2008
