This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1t6l
From Proteopedia
(New page: 200px<br /><applet load="1t6l" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t6l, resolution 1.85Å" /> '''Crystal Structure of...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1t6l.jpg|left|200px]]<br /><applet load="1t6l" size=" | + | [[Image:1t6l.jpg|left|200px]]<br /><applet load="1t6l" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1t6l, resolution 1.85Å" /> | caption="1t6l, resolution 1.85Å" /> | ||
'''Crystal Structure of the Human Cytomegalovirus DNA Polymerase Subunit, UL44'''<br /> | '''Crystal Structure of the Human Cytomegalovirus DNA Polymerase Subunit, UL44'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The human cytomegalovirus DNA polymerase consists of a catalytic subunit, UL54, and a presumed processivity factor, UL44. We have solved the crystal | + | The human cytomegalovirus DNA polymerase consists of a catalytic subunit, UL54, and a presumed processivity factor, UL44. We have solved the crystal structure of residues 1-290 of UL44 to 1.85 A resolution by multiwavelength anomalous dispersion. The structure reveals a dimer of UL44 in the shape of a C clamp. Each monomer of UL44 shares its overall fold with other processivity factors, including herpes simplex virus UL42, which is a monomer that binds DNA directly, and the sliding clamp, PCNA, which is a trimer that surrounds DNA, although these proteins share no obvious sequence homology. Analytical ultracentrifugation and gel filtration measurements demonstrated that UL44 also forms a dimer in solution, and substitution of large hydrophobic residues along the homodimer interface with alanine disrupted dimerization and decreased DNA binding. UL44 represents a hybrid processivity factor as it binds DNA directly like UL42, but forms a C clamp that may surround DNA like PCNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1T6L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_5 Human herpesvirus 5]. Full crystallographic information is available from [http:// | + | 1T6L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_5 Human herpesvirus 5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T6L OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Human herpesvirus 5]] | [[Category: Human herpesvirus 5]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Appleton, B | + | [[Category: Appleton, B A.]] |
| - | [[Category: Coen, D | + | [[Category: Coen, D M.]] |
| - | [[Category: Filman, D | + | [[Category: Filman, D J.]] |
| - | [[Category: Hogle, J | + | [[Category: Hogle, J M.]] |
[[Category: Loregian, A.]] | [[Category: Loregian, A.]] | ||
[[Category: processivity fold]] | [[Category: processivity fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:10:30 2008'' |
Revision as of 13:10, 21 February 2008
|
Crystal Structure of the Human Cytomegalovirus DNA Polymerase Subunit, UL44
Overview
The human cytomegalovirus DNA polymerase consists of a catalytic subunit, UL54, and a presumed processivity factor, UL44. We have solved the crystal structure of residues 1-290 of UL44 to 1.85 A resolution by multiwavelength anomalous dispersion. The structure reveals a dimer of UL44 in the shape of a C clamp. Each monomer of UL44 shares its overall fold with other processivity factors, including herpes simplex virus UL42, which is a monomer that binds DNA directly, and the sliding clamp, PCNA, which is a trimer that surrounds DNA, although these proteins share no obvious sequence homology. Analytical ultracentrifugation and gel filtration measurements demonstrated that UL44 also forms a dimer in solution, and substitution of large hydrophobic residues along the homodimer interface with alanine disrupted dimerization and decreased DNA binding. UL44 represents a hybrid processivity factor as it binds DNA directly like UL42, but forms a C clamp that may surround DNA like PCNA.
About this Structure
1T6L is a Single protein structure of sequence from Human herpesvirus 5. Full crystallographic information is available from OCA.
Reference
The cytomegalovirus DNA polymerase subunit UL44 forms a C clamp-shaped dimer., Appleton BA, Loregian A, Filman DJ, Coen DM, Hogle JM, Mol Cell. 2004 Jul 23;15(2):233-44. PMID:15260974
Page seeded by OCA on Thu Feb 21 15:10:30 2008
