1t7p
From Proteopedia
(New page: 200px<br /><applet load="1t7p" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t7p, resolution 2.2Å" /> '''T7 DNA POLYMERASE COM...) |
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| - | [[Image:1t7p.gif|left|200px]]<br /><applet load="1t7p" size=" | + | [[Image:1t7p.gif|left|200px]]<br /><applet load="1t7p" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1t7p, resolution 2.2Å" /> | caption="1t7p, resolution 2.2Å" /> | ||
'''T7 DNA POLYMERASE COMPLEXED TO DNA PRIMER/TEMPLATE,A NUCLEOSIDE TRIPHOSPHATE, AND ITS PROCESSIVITY FACTOR THIOREDOXIN'''<br /> | '''T7 DNA POLYMERASE COMPLEXED TO DNA PRIMER/TEMPLATE,A NUCLEOSIDE TRIPHOSPHATE, AND ITS PROCESSIVITY FACTOR THIOREDOXIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | DNA polymerases change their specificity for nucleotide substrates with | + | DNA polymerases change their specificity for nucleotide substrates with each catalytic cycle, while achieving error frequencies in the range of 10(-5) to 10(-6). Here we present a 2.2 A crystal structure of the replicative DNA polymerase from bacteriophage T7 complexed with a primer-template and a nucleoside triphosphate in the polymerase active site. The structure illustrates how nucleotides are selected in a template-directed manner, and provides a structural basis for a metal-assisted mechanism of phosphoryl transfer by a large group of related polymerases. |
==About this Structure== | ==About this Structure== | ||
| - | 1T7P is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with MG and DG3 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http:// | + | 1T7P is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacteriophage_t7 Bacteriophage t7] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=DG3:'>DG3</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7P OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Doublie, S.]] | [[Category: Doublie, S.]] | ||
[[Category: Ellenberger, T.]] | [[Category: Ellenberger, T.]] | ||
| - | [[Category: Long, A | + | [[Category: Long, A M.]] |
| - | [[Category: Richardson, C | + | [[Category: Richardson, C C.]] |
[[Category: Tabor, S.]] | [[Category: Tabor, S.]] | ||
[[Category: DG3]] | [[Category: DG3]] | ||
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[[Category: thioredoxin]] | [[Category: thioredoxin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:10:45 2008'' |
Revision as of 13:10, 21 February 2008
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T7 DNA POLYMERASE COMPLEXED TO DNA PRIMER/TEMPLATE,A NUCLEOSIDE TRIPHOSPHATE, AND ITS PROCESSIVITY FACTOR THIOREDOXIN
Overview
DNA polymerases change their specificity for nucleotide substrates with each catalytic cycle, while achieving error frequencies in the range of 10(-5) to 10(-6). Here we present a 2.2 A crystal structure of the replicative DNA polymerase from bacteriophage T7 complexed with a primer-template and a nucleoside triphosphate in the polymerase active site. The structure illustrates how nucleotides are selected in a template-directed manner, and provides a structural basis for a metal-assisted mechanism of phosphoryl transfer by a large group of related polymerases.
About this Structure
1T7P is a Protein complex structure of sequences from Bacteriophage t7 and Escherichia coli with and as ligands. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.
Reference
Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 A resolution., Doublie S, Tabor S, Long AM, Richardson CC, Ellenberger T, Nature. 1998 Jan 15;391(6664):251-8. PMID:9440688
Page seeded by OCA on Thu Feb 21 15:10:45 2008
Categories: Bacteriophage t7 | DNA-directed DNA polymerase | Escherichia coli | Protein complex | Doublie, S. | Ellenberger, T. | Long, A M. | Richardson, C C. | Tabor, S. | DG3 | MG | Complex (hydrolase/electron transport/dna) | Dna replication | Nucleotidyl transferase | Processivity factor | Sequencing | T7 dna polymerase | Thioredoxin
