1t8k
From Proteopedia
(New page: 200px<br /><applet load="1t8k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t8k, resolution 1.10Å" /> '''Crystal Structure of...) |
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| - | [[Image:1t8k.jpg|left|200px]]<br /><applet load="1t8k" size=" | + | [[Image:1t8k.jpg|left|200px]]<br /><applet load="1t8k" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1t8k, resolution 1.10Å" /> | caption="1t8k, resolution 1.10Å" /> | ||
'''Crystal Structure of apo acyl carrier protein from E. coli'''<br /> | '''Crystal Structure of apo acyl carrier protein from E. coli'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A topic of current interest is engineering surface mutations in order to | + | A topic of current interest is engineering surface mutations in order to improve the success rate of protein crystallization. This report explores the possibility of using metal-ion-mediated crystal-packing interactions to facilitate rational design. Escherichia coli apo acyl carrier protein was chosen as a test case because of its high content of negatively charged carboxylates suitable for metal binding with moderate affinity. The protein was successfully crystallized in the presence of zinc ions. The crystal structure was determined to 1.1 A resolution with MAD phasing using anomalous signals from the co-crystallized Zn(2+) ions. The case study suggested an integrated strategy for crystallization and structure solution of proteins via engineering surface Asp and Glu mutants, crystallizing them in the presence of metal ions such as Zn(2+) and solving the structures using anomalous signals. |
==About this Structure== | ==About this Structure== | ||
| - | 1T8K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN and IMD as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1T8K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=IMD:'>IMD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Janson, C | + | [[Category: Janson, C A.]] |
[[Category: Qiu, X.]] | [[Category: Qiu, X.]] | ||
[[Category: IMD]] | [[Category: IMD]] | ||
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[[Category: acp]] | [[Category: acp]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:11:03 2008'' |
Revision as of 13:11, 21 February 2008
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Crystal Structure of apo acyl carrier protein from E. coli
Overview
A topic of current interest is engineering surface mutations in order to improve the success rate of protein crystallization. This report explores the possibility of using metal-ion-mediated crystal-packing interactions to facilitate rational design. Escherichia coli apo acyl carrier protein was chosen as a test case because of its high content of negatively charged carboxylates suitable for metal binding with moderate affinity. The protein was successfully crystallized in the presence of zinc ions. The crystal structure was determined to 1.1 A resolution with MAD phasing using anomalous signals from the co-crystallized Zn(2+) ions. The case study suggested an integrated strategy for crystallization and structure solution of proteins via engineering surface Asp and Glu mutants, crystallizing them in the presence of metal ions such as Zn(2+) and solving the structures using anomalous signals.
About this Structure
1T8K is a Single protein structure of sequence from Escherichia coli with and as ligands. Full crystallographic information is available from OCA.
Reference
Structure of apo acyl carrier protein and a proposal to engineer protein crystallization through metal ions., Qiu X, Janson CA, Acta Crystallogr D Biol Crystallogr. 2004 Sep;60(Pt 9):1545-54. Epub 2004, Aug 26. PMID:15333924
Page seeded by OCA on Thu Feb 21 15:11:03 2008
Categories: Escherichia coli | Single protein | Janson, C A. | Qiu, X. | IMD | ZN | Acp
