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1t98
From Proteopedia
(New page: 200px<br /><applet load="1t98" size="450" color="white" frame="true" align="right" spinBox="true" caption="1t98, resolution 2.90Å" /> '''Crystal Structure of...) |
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| - | [[Image:1t98.gif|left|200px]]<br /><applet load="1t98" size=" | + | [[Image:1t98.gif|left|200px]]<br /><applet load="1t98" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1t98, resolution 2.90Å" /> | caption="1t98, resolution 2.90Å" /> | ||
'''Crystal Structure of MukF(1-287)'''<br /> | '''Crystal Structure of MukF(1-287)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Escherichia coli MukB, MukE, and MukF proteins form a bacterial | + | The Escherichia coli MukB, MukE, and MukF proteins form a bacterial condensin (MukBEF) that contributes to chromosome management by compacting DNA. MukB is an ATPase and DNA-binding protein of the SMC superfamily; however, the structure and function of non-SMC components, such as MukF, have been less forthcoming. Here, we report the crystal structure of the N-terminal 287 amino acids of MukF at 2.9 A resolution. This region folds into a winged-helix domain and an extended coiled-coil domain that self-associate to form a stable, doubly domain-swapped dimer. Protein dissection and affinity purification data demonstrate that the region of MukF C-terminal to this fragment binds to MukE and MukB. Our findings, together with sequence analyses, indicate that MukF is a kleisin subunit for E. coli condensin and suggest a means by which it may organize the MukBEF assembly. |
==About this Structure== | ==About this Structure== | ||
| - | 1T98 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1T98 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T98 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Berger, J | + | [[Category: Berger, J M.]] |
[[Category: Fennell-Fezzie, R.]] | [[Category: Fennell-Fezzie, R.]] | ||
[[Category: coiled coil]] | [[Category: coiled coil]] | ||
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[[Category: winged helix]] | [[Category: winged helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:11:14 2008'' |
Revision as of 13:11, 21 February 2008
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Crystal Structure of MukF(1-287)
Overview
The Escherichia coli MukB, MukE, and MukF proteins form a bacterial condensin (MukBEF) that contributes to chromosome management by compacting DNA. MukB is an ATPase and DNA-binding protein of the SMC superfamily; however, the structure and function of non-SMC components, such as MukF, have been less forthcoming. Here, we report the crystal structure of the N-terminal 287 amino acids of MukF at 2.9 A resolution. This region folds into a winged-helix domain and an extended coiled-coil domain that self-associate to form a stable, doubly domain-swapped dimer. Protein dissection and affinity purification data demonstrate that the region of MukF C-terminal to this fragment binds to MukE and MukB. Our findings, together with sequence analyses, indicate that MukF is a kleisin subunit for E. coli condensin and suggest a means by which it may organize the MukBEF assembly.
About this Structure
1T98 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
The MukF subunit of Escherichia coli condensin: architecture and functional relationship to kleisins., Fennell-Fezzie R, Gradia SD, Akey D, Berger JM, EMBO J. 2005 Jun 1;24(11):1921-30. Epub 2005 May 19. PMID:15902272
Page seeded by OCA on Thu Feb 21 15:11:14 2008
