1tbl
From Proteopedia
(New page: 200px<br /><applet load="1tbl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tbl, resolution 3.10Å" /> '''H141N mutant of rat ...) |
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| - | [[Image:1tbl.gif|left|200px]]<br /><applet load="1tbl" size=" | + | [[Image:1tbl.gif|left|200px]]<br /><applet load="1tbl" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1tbl, resolution 3.10Å" /> | caption="1tbl, resolution 3.10Å" /> | ||
'''H141N mutant of rat liver arginase I'''<br /> | '''H141N mutant of rat liver arginase I'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Rat liver arginase (arginase I) is potently inactivated by diethyl | + | Rat liver arginase (arginase I) is potently inactivated by diethyl pyrocarbonate, with a second-order rate constant of 113M(-1)s(-1) for the inactivation process at pH 7.0, 25 degrees C. Partial protection from inactivation is provided by the product of the reaction, l-ornithine, while nearly complete protection is afforded by the inhibitor pair, l-ornithine and borate. The role of H141 has been probed by mutagenesis, chemical modulation, and X-ray diffraction. The hyper-reactivity of H141 towards diethyl pyrocarbonate can be explained by its proximity to E277. A proton shuttling role for H141 is supported by its conformational mobility observed among the known arginase structures. H141 is proposed to serve as an acid/base catalyst, deprotonating the metal-bridging water molecule to generate the metal-bridging hydroxide nucleophile, and by protonating the amino group of the product to facilitate its departure. |
==About this Structure== | ==About this Structure== | ||
| - | 1TBL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with MN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Arginase Arginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.1 3.5.3.1] Full crystallographic information is available from [http:// | + | 1TBL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=MN:'>MN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Arginase Arginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.3.1 3.5.3.1] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TBL OCA]. |
==Reference== | ==Reference== | ||
| Line 14: | Line 14: | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ash, D | + | [[Category: Ash, D E.]] |
[[Category: Cama, E.]] | [[Category: Cama, E.]] | ||
| - | [[Category: Christianson, D | + | [[Category: Christianson, D W.]] |
| - | [[Category: Cox, J | + | [[Category: Cox, J D.]] |
[[Category: MN]] | [[Category: MN]] | ||
[[Category: arginase i]] | [[Category: arginase i]] | ||
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[[Category: h141n mutant]] | [[Category: h141n mutant]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:11:53 2008'' |
Revision as of 13:11, 21 February 2008
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H141N mutant of rat liver arginase I
Overview
Rat liver arginase (arginase I) is potently inactivated by diethyl pyrocarbonate, with a second-order rate constant of 113M(-1)s(-1) for the inactivation process at pH 7.0, 25 degrees C. Partial protection from inactivation is provided by the product of the reaction, l-ornithine, while nearly complete protection is afforded by the inhibitor pair, l-ornithine and borate. The role of H141 has been probed by mutagenesis, chemical modulation, and X-ray diffraction. The hyper-reactivity of H141 towards diethyl pyrocarbonate can be explained by its proximity to E277. A proton shuttling role for H141 is supported by its conformational mobility observed among the known arginase structures. H141 is proposed to serve as an acid/base catalyst, deprotonating the metal-bridging water molecule to generate the metal-bridging hydroxide nucleophile, and by protonating the amino group of the product to facilitate its departure.
About this Structure
1TBL is a Single protein structure of sequence from Rattus norvegicus with as ligand. Active as Arginase, with EC number 3.5.3.1 Full crystallographic information is available from OCA.
Reference
Probing the role of the hyper-reactive histidine residue of arginase., Colleluori DM, Reczkowski RS, Emig FA, Cama E, Cox JD, Scolnick LR, Compher K, Jude K, Han S, Viola RE, Christianson DW, Ash DE, Arch Biochem Biophys. 2005 Dec 1;444(1):15-26. Epub 2005 Oct 13. PMID:16266687
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