1tbd
From Proteopedia
(New page: 200px<br /> <applet load="1tbd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tbd" /> '''SOLUTION STRUCTURE OF THE ORIGIN DNA BINDIN...) |
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'''SOLUTION STRUCTURE OF THE ORIGIN DNA BINDING DOMAIN OF SV40 T-ANTIGEN, NMR, MINIMIZED AVERAGE STRUCTURE'''<br /> | '''SOLUTION STRUCTURE OF THE ORIGIN DNA BINDING DOMAIN OF SV40 T-ANTIGEN, NMR, MINIMIZED AVERAGE STRUCTURE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the domain from simian virus 40 (SV40) large T-antigen | + | The structure of the domain from simian virus 40 (SV40) large T-antigen that binds to the SV40 origin of DNA replication (T-ag-OBD131-260) has been determined by nuclear magnetic resonance spectroscopy. The overall fold, consisting of a central five-stranded antiparallel beta-sheet flanked by two alpha-helices on one side and one alpha-helix and one 3(10)-helix on the other, is a new one. Previous mutational analyses have identified two elements, termed A (approximately 152-155) and B2 (203-207), as essential for origin-specific recognition. These elements form two closely juxtaposed loops that define a continuous surface on the protein. The addition of a duplex oligonucleotide containing the origin recognition pentanucleotide GAGGC induces chemical shift changes and slows amide proton exchange in resonances from this region, indicating that this surface directly contacts the DNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1TBD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. The following page contains interesting information on the relation of 1TBD with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb47_1.html Simian Virus 40]]. Full crystallographic information is available from [http:// | + | 1TBD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Simian_virus_40 Simian virus 40]. The following page contains interesting information on the relation of 1TBD with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb47_1.html Simian Virus 40]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TBD OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Simian virus 40]] | [[Category: Simian virus 40]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bachovchin, W | + | [[Category: Bachovchin, W W.]] |
| - | [[Category: Bullock, P | + | [[Category: Bullock, P A.]] |
[[Category: Luo, X.]] | [[Category: Luo, X.]] | ||
| - | [[Category: Sanford, D | + | [[Category: Sanford, D G.]] |
[[Category: dna-binding protein]] | [[Category: dna-binding protein]] | ||
[[Category: replication origin binding domain]] | [[Category: replication origin binding domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:11:52 2008'' |
Revision as of 13:11, 21 February 2008
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SOLUTION STRUCTURE OF THE ORIGIN DNA BINDING DOMAIN OF SV40 T-ANTIGEN, NMR, MINIMIZED AVERAGE STRUCTURE
Overview
The structure of the domain from simian virus 40 (SV40) large T-antigen that binds to the SV40 origin of DNA replication (T-ag-OBD131-260) has been determined by nuclear magnetic resonance spectroscopy. The overall fold, consisting of a central five-stranded antiparallel beta-sheet flanked by two alpha-helices on one side and one alpha-helix and one 3(10)-helix on the other, is a new one. Previous mutational analyses have identified two elements, termed A (approximately 152-155) and B2 (203-207), as essential for origin-specific recognition. These elements form two closely juxtaposed loops that define a continuous surface on the protein. The addition of a duplex oligonucleotide containing the origin recognition pentanucleotide GAGGC induces chemical shift changes and slows amide proton exchange in resonances from this region, indicating that this surface directly contacts the DNA.
About this Structure
1TBD is a Single protein structure of sequence from Simian virus 40. The following page contains interesting information on the relation of 1TBD with [Simian Virus 40]. Full crystallographic information is available from OCA.
Reference
Solution structure of the origin DNA-binding domain of SV40 T-antigen., Luo X, Sanford DG, Bullock PA, Bachovchin WW, Nat Struct Biol. 1996 Dec;3(12):1034-9. PMID:8946857
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