1tez
From Proteopedia
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==Overview== | ==Overview== | ||
| - | DNA photolyases use light energy to repair DNA that comprises | + | DNA photolyases use light energy to repair DNA that comprises ultraviolet-induced lesions such as the cis-syn cyclobutane pyrimidine dimers (CPDs). Here we report the crystal structure of a DNA photolyase bound to duplex DNA that is bent by 50 degrees and comprises a synthetic CPD lesion. This CPD lesion is flipped into the active site and split there into two thymines by synchrotron radiation at 100 K. Although photolyases catalyze blue light-driven CPD cleavage only above 200 K, this structure apparently mimics a structural substate during light-driven DNA repair in which back-flipping of the thymines into duplex DNA has not yet taken place. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Synechococcus sp.]] | [[Category: Synechococcus sp.]] | ||
[[Category: Carell, T.]] | [[Category: Carell, T.]] | ||
| - | [[Category: Essen, L | + | [[Category: Essen, L O.]] |
[[Category: Klar, T.]] | [[Category: Klar, T.]] | ||
[[Category: Mees, A.]] | [[Category: Mees, A.]] | ||
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[[Category: photolyase; dna repair; light-driven electron transfer]] | [[Category: photolyase; dna repair; light-driven electron transfer]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:12:52 2008'' |
Revision as of 13:12, 21 February 2008
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COMPLEX BETWEEN DNA AND THE DNA PHOTOLYASE FROM ANACYSTIS NIDULANS
Overview
DNA photolyases use light energy to repair DNA that comprises ultraviolet-induced lesions such as the cis-syn cyclobutane pyrimidine dimers (CPDs). Here we report the crystal structure of a DNA photolyase bound to duplex DNA that is bent by 50 degrees and comprises a synthetic CPD lesion. This CPD lesion is flipped into the active site and split there into two thymines by synchrotron radiation at 100 K. Although photolyases catalyze blue light-driven CPD cleavage only above 200 K, this structure apparently mimics a structural substate during light-driven DNA repair in which back-flipping of the thymines into duplex DNA has not yet taken place.
About this Structure
1TEZ is a Single protein structure of sequence from Synechococcus sp. with , and as ligands. Active as Deoxyribodipyrimidine photo-lyase, with EC number 4.1.99.3 Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Crystal structure of a photolyase bound to a CPD-like DNA lesion after in situ repair., Mees A, Klar T, Gnau P, Hennecke U, Eker AP, Carell T, Essen LO, Science. 2004 Dec 3;306(5702):1789-93. PMID:15576622
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