1tf7
From Proteopedia
(New page: 200px<br /><applet load="1tf7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tf7, resolution 2.8Å" /> '''Crystal Structure of ...) |
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| - | [[Image:1tf7.jpg|left|200px]]<br /><applet load="1tf7" size=" | + | [[Image:1tf7.jpg|left|200px]]<br /><applet load="1tf7" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1tf7, resolution 2.8Å" /> | caption="1tf7, resolution 2.8Å" /> | ||
'''Crystal Structure of Circadian Clock Protein KaiC'''<br /> | '''Crystal Structure of Circadian Clock Protein KaiC'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Circadian (daily) biological clocks express characteristics that are | + | Circadian (daily) biological clocks express characteristics that are difficult to explain by known biochemical mechanisms, and will ultimately require characterizing the structures, functions, and interactions of their molecular components. KaiC is an essential circadian protein in cyanobacteria that forms the core of the KaiABC clock protein complex. We report the crystal structure of the KaiC homohexameric complex at 2.8 A resolution. The structure resembles a double doughnut with a central pore that is partially sealed at one end. The crystal structure reveals ATP binding, inter-subunit organization, a scaffold for Kai-protein complex formation, the location of critical KaiC mutations, and evolutionary relationships to other proteins. A key auto-phosphorylation site on KaiC (T432) is identified from the crystal structure, and mutation of this residue abolishes circadian rhythmicity. The crystal structure of KaiC will be essential for understanding this circadian clockwork and for establishing its links to global gene expression. |
==About this Structure== | ==About this Structure== | ||
| - | 1TF7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.] with ATP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1TF7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.] with <scene name='pdbligand=ATP:'>ATP</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TF7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Synechococcus sp.]] | [[Category: Synechococcus sp.]] | ||
[[Category: Egli, M.]] | [[Category: Egli, M.]] | ||
| - | [[Category: Johnson, C | + | [[Category: Johnson, C H.]] |
[[Category: Mori, T.]] | [[Category: Mori, T.]] | ||
[[Category: Pattanayek, R.]] | [[Category: Pattanayek, R.]] | ||
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[[Category: homohexamer]] | [[Category: homohexamer]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:12:55 2008'' |
Revision as of 13:13, 21 February 2008
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Crystal Structure of Circadian Clock Protein KaiC
Overview
Circadian (daily) biological clocks express characteristics that are difficult to explain by known biochemical mechanisms, and will ultimately require characterizing the structures, functions, and interactions of their molecular components. KaiC is an essential circadian protein in cyanobacteria that forms the core of the KaiABC clock protein complex. We report the crystal structure of the KaiC homohexameric complex at 2.8 A resolution. The structure resembles a double doughnut with a central pore that is partially sealed at one end. The crystal structure reveals ATP binding, inter-subunit organization, a scaffold for Kai-protein complex formation, the location of critical KaiC mutations, and evolutionary relationships to other proteins. A key auto-phosphorylation site on KaiC (T432) is identified from the crystal structure, and mutation of this residue abolishes circadian rhythmicity. The crystal structure of KaiC will be essential for understanding this circadian clockwork and for establishing its links to global gene expression.
About this Structure
1TF7 is a Single protein structure of sequence from Synechococcus sp. with as ligand. Full crystallographic information is available from OCA.
Reference
Visualizing a circadian clock protein: crystal structure of KaiC and functional insights., Pattanayek R, Wang J, Mori T, Xu Y, Johnson CH, Egli M, Mol Cell. 2004 Aug 13;15(3):375-88. PMID:15304218
Page seeded by OCA on Thu Feb 21 15:12:55 2008
Categories: Single protein | Synechococcus sp. | Egli, M. | Johnson, C H. | Mori, T. | Pattanayek, R. | Wang, J. | Xu, Y. | ATP | Hexamer | Homohexamer
