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1tgl

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(New page: 200px<br /><applet load="1tgl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tgl, resolution 1.9&Aring;" /> '''A SERINE PROTEASE TRI...)
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'''A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTRE OF A TRIACYLGLYCEROL LIPASE'''<br />
'''A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTRE OF A TRIACYLGLYCEROL LIPASE'''<br />
==Overview==
==Overview==
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True lipases attach triacylglycerols and act at an oil-water interface;, they constitute a ubiquitous group of enzymes catalysing a wide variety of, reactions, many with industrial potential. But so far the, three-dimensional structure has not been reported for any lipase. Here we, report the X-ray structure of the Mucor miehei triglyceride lipase and, describe the atomic model obtained at 3.1 A resolution and refined to 1.9, A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with, an active serine buried under a short helical fragment of a long surface, loop.
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True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
==About this Structure==
==About this Structure==
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1TGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1TGL OCA].
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1TGL is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhizomucor_miehei Rhizomucor miehei]. Active as [http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TGL OCA].
==Reference==
==Reference==
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[[Category: Triacylglycerol lipase]]
[[Category: Triacylglycerol lipase]]
[[Category: Brady, L.]]
[[Category: Brady, L.]]
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[[Category: Brzozowski, A.M.]]
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[[Category: Brzozowski, A M.]]
[[Category: Christiansen, L.]]
[[Category: Christiansen, L.]]
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[[Category: Derewenda, Z.S.]]
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[[Category: Derewenda, Z S.]]
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[[Category: Dodson, E.J.]]
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[[Category: Dodson, E J.]]
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[[Category: Dodson, G.G.]]
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[[Category: Dodson, G G.]]
[[Category: Huge-Jensen, B.]]
[[Category: Huge-Jensen, B.]]
[[Category: Norskov, L.]]
[[Category: Norskov, L.]]
[[Category: Thim, L.]]
[[Category: Thim, L.]]
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[[Category: Tolley, S.P.]]
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[[Category: Tolley, S P.]]
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[[Category: Turkenburg, J.P.]]
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[[Category: Turkenburg, J P.]]
[[Category: hydrolase(carboxylic esterase)]]
[[Category: hydrolase(carboxylic esterase)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:13:17 2008''

Revision as of 13:13, 21 February 2008

Image:1tgl.jpg

1tgl, resolution 1.9Å

Drag the structure with the mouse to rotate

A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTRE OF A TRIACYLGLYCEROL LIPASE

Overview

True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.

About this Structure

1TGL is a Single protein structure of sequence from Rhizomucor miehei. Active as Triacylglycerol lipase, with EC number 3.1.1.3 Full crystallographic information is available from OCA.

Reference

A serine protease triad forms the catalytic centre of a triacylglycerol lipase., Brady L, Brzozowski AM, Derewenda ZS, Dodson E, Dodson G, Tolley S, Turkenburg JP, Christiansen L, Huge-Jensen B, Norskov L, et al., Nature. 1990 Feb 22;343(6260):767-70. PMID:2304552

Page seeded by OCA on Thu Feb 21 15:13:17 2008

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