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1thd
From Proteopedia
(New page: 200px<br /><applet load="1thd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1thd" /> '''COMPLEX ORGANIZATION OF DENGUE VIRUS E PROTE...) |
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| - | [[Image:1thd.gif|left|200px]]<br /><applet load="1thd" size=" | + | [[Image:1thd.gif|left|200px]]<br /><applet load="1thd" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1thd" /> | caption="1thd" /> | ||
'''COMPLEX ORGANIZATION OF DENGUE VIRUS E PROTEIN AS REVEALED BY 9.5 ANGSTROM CRYO-EM RECONSTRUCTION'''<br /> | '''COMPLEX ORGANIZATION OF DENGUE VIRUS E PROTEIN AS REVEALED BY 9.5 ANGSTROM CRYO-EM RECONSTRUCTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Dengue virus, a member of the Flaviviridae family, has a surface composed | + | Dengue virus, a member of the Flaviviridae family, has a surface composed of 180 copies each of the envelope (E) glycoprotein and the membrane (M) protein. The crystal structure of an N-terminal fragment of E has been determined and compared with a previously described structure. The primary difference between these structures is a 10 degrees rotation about a hinge relating the fusion domain DII to domains DI and DIII. These two rigid body components were used for independent fitting of E into the cryo-electron microscopy maps of both immature and mature dengue viruses. The fitted E structures in these two particles showed a difference of 27 degrees between the two components. Comparison of the E structure in its postfusion state with that in the immature and mature virions shows a rotation approximately around the same hinge. Flexibility of E is apparently a functional requirement for assembly and infection of flaviviruses. |
==About this Structure== | ==About this Structure== | ||
| - | 1THD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dengue_virus_type_3 Dengue virus type 3]. Full crystallographic information is available from [http:// | + | 1THD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Dengue_virus_type_3 Dengue virus type 3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1THD OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Dengue virus type 3]] | [[Category: Dengue virus type 3]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Baker, T | + | [[Category: Baker, T S.]] |
[[Category: Clements, D.]] | [[Category: Clements, D.]] | ||
| - | [[Category: Kuhn, R | + | [[Category: Kuhn, R J.]] |
[[Category: Ogata, S.]] | [[Category: Ogata, S.]] | ||
| - | [[Category: Rossmann, M | + | [[Category: Rossmann, M G.]] |
| - | [[Category: Strauss, J | + | [[Category: Strauss, J H.]] |
[[Category: Zhang, W.]] | [[Category: Zhang, W.]] | ||
[[Category: Zhang, Y.]] | [[Category: Zhang, Y.]] | ||
| Line 29: | Line 29: | ||
[[Category: icosahedral virus]] | [[Category: icosahedral virus]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:13:31 2008'' |
Revision as of 13:13, 21 February 2008
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COMPLEX ORGANIZATION OF DENGUE VIRUS E PROTEIN AS REVEALED BY 9.5 ANGSTROM CRYO-EM RECONSTRUCTION
Overview
Dengue virus, a member of the Flaviviridae family, has a surface composed of 180 copies each of the envelope (E) glycoprotein and the membrane (M) protein. The crystal structure of an N-terminal fragment of E has been determined and compared with a previously described structure. The primary difference between these structures is a 10 degrees rotation about a hinge relating the fusion domain DII to domains DI and DIII. These two rigid body components were used for independent fitting of E into the cryo-electron microscopy maps of both immature and mature dengue viruses. The fitted E structures in these two particles showed a difference of 27 degrees between the two components. Comparison of the E structure in its postfusion state with that in the immature and mature virions shows a rotation approximately around the same hinge. Flexibility of E is apparently a functional requirement for assembly and infection of flaviviruses.
About this Structure
1THD is a Single protein structure of sequence from Dengue virus type 3. Full crystallographic information is available from OCA.
Reference
Conformational changes of the flavivirus E glycoprotein., Zhang Y, Zhang W, Ogata S, Clements D, Strauss JH, Baker TS, Kuhn RJ, Rossmann MG, Structure. 2004 Sep;12(9):1607-18. PMID:15341726
Page seeded by OCA on Thu Feb 21 15:13:31 2008
