1tif
From Proteopedia
(New page: 200px<br /><applet load="1tif" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tif, resolution 1.8Å" /> '''TRANSLATION INITIATIO...) |
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| - | [[Image:1tif.gif|left|200px]]<br /><applet load="1tif" size=" | + | [[Image:1tif.gif|left|200px]]<br /><applet load="1tif" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1tif, resolution 1.8Å" /> | caption="1tif, resolution 1.8Å" /> | ||
'''TRANSLATION INITIATION FACTOR 3 N-TERMINAL DOMAIN'''<br /> | '''TRANSLATION INITIATION FACTOR 3 N-TERMINAL DOMAIN'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structures of the two domains of translational initiation factor IF3 | + | The structures of the two domains of translational initiation factor IF3 from Bacillus stearothermophilus have been solved by X-ray crystallography using single wavelength anomalous scattering and multiwavelength anomalous diffraction. Each of the two domains has an alpha/beta topology, with an exposed beta-sheet that is reminiscent of several ribosomal and other RNA binding proteins. An alpha-helix that protrudes out from the body of the N-terminal domain towards the C-terminal domain suggests that IF3 consists of two RNA binding domains connected by an alpha-helix and that it may bridge two regions of the ribosome. This represents the first high resolution structural information on a translational initiation factor. |
==About this Structure== | ==About this Structure== | ||
| - | 1TIF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with PBM and CL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1TIF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus] with <scene name='pdbligand=PBM:'>PBM</scene> and <scene name='pdbligand=CL:'>CL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: if3 n-terminal domain]] | [[Category: if3 n-terminal domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:13:49 2008'' |
Revision as of 13:13, 21 February 2008
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TRANSLATION INITIATION FACTOR 3 N-TERMINAL DOMAIN
Overview
The structures of the two domains of translational initiation factor IF3 from Bacillus stearothermophilus have been solved by X-ray crystallography using single wavelength anomalous scattering and multiwavelength anomalous diffraction. Each of the two domains has an alpha/beta topology, with an exposed beta-sheet that is reminiscent of several ribosomal and other RNA binding proteins. An alpha-helix that protrudes out from the body of the N-terminal domain towards the C-terminal domain suggests that IF3 consists of two RNA binding domains connected by an alpha-helix and that it may bridge two regions of the ribosome. This represents the first high resolution structural information on a translational initiation factor.
About this Structure
1TIF is a Single protein structure of sequence from Geobacillus stearothermophilus with and as ligands. Full crystallographic information is available from OCA.
Reference
X-ray crystallography shows that translational initiation factor IF3 consists of two compact alpha/beta domains linked by an alpha-helix., Biou V, Shu F, Ramakrishnan V, EMBO J. 1995 Aug 15;14(16):4056-64. PMID:7664745
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