1tip
From Proteopedia
(New page: 200px<br /><applet load="1tip" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tip, resolution 2.2Å" /> '''THE BISPHOSPHATASE DO...) |
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| - | [[Image:1tip.jpg|left|200px]]<br /><applet load="1tip" size=" | + | [[Image:1tip.jpg|left|200px]]<br /><applet load="1tip" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1tip, resolution 2.2Å" /> | caption="1tip, resolution 2.2Å" /> | ||
'''THE BISPHOSPHATASE DOMAIN OF THE BIFUNCTIONAL RAT LIVER 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE'''<br /> | '''THE BISPHOSPHATASE DOMAIN OF THE BIFUNCTIONAL RAT LIVER 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of the fructose-2,6-bisphosphatase domain trapped | + | The crystal structure of the fructose-2,6-bisphosphatase domain trapped during the reaction reveal a phosphorylated His 258, and a water molecule immobilized by the product, fructose-6-phosphate. The geometry suggests that the dephosphorylation step requires prior removal of the product for an 'associative in-line' phosphoryl transfer to the catalytic water. |
==About this Structure== | ==About this Structure== | ||
| - | 1TIP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with F6P as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Fructose-2,6-bisphosphate_2-phosphatase Fructose-2,6-bisphosphate 2-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.46 3.1.3.46] Full crystallographic information is available from [http:// | + | 1TIP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=F6P:'>F6P</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Fructose-2,6-bisphosphate_2-phosphatase Fructose-2,6-bisphosphate 2-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.46 3.1.3.46] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TIP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Banaszak, L | + | [[Category: Banaszak, L J.]] |
| - | [[Category: Lange, A | + | [[Category: Lange, A J.]] |
| - | [[Category: Lee, Y | + | [[Category: Lee, Y H.]] |
| - | [[Category: Levitt, D | + | [[Category: Levitt, D G.]] |
| - | [[Category: Ogata, C | + | [[Category: Ogata, C M.]] |
| - | [[Category: Olson, T | + | [[Category: Olson, T W.]] |
[[Category: F6P]] | [[Category: F6P]] | ||
[[Category: alternative splicing]] | [[Category: alternative splicing]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:13:57 2008'' |
Revision as of 13:13, 21 February 2008
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THE BISPHOSPHATASE DOMAIN OF THE BIFUNCTIONAL RAT LIVER 6-PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOSPHATASE
Overview
The crystal structure of the fructose-2,6-bisphosphatase domain trapped during the reaction reveal a phosphorylated His 258, and a water molecule immobilized by the product, fructose-6-phosphate. The geometry suggests that the dephosphorylation step requires prior removal of the product for an 'associative in-line' phosphoryl transfer to the catalytic water.
About this Structure
1TIP is a Single protein structure of sequence from Rattus norvegicus with as ligand. Active as Fructose-2,6-bisphosphate 2-phosphatase, with EC number 3.1.3.46 Full crystallographic information is available from OCA.
Reference
Crystal structure of a trapped phosphoenzyme during a catalytic reaction., Lee YH, Olson TW, Ogata CM, Levitt DG, Banaszak LJ, Lange AJ, Nat Struct Biol. 1997 Aug;4(8):615-8. PMID:9253407
Page seeded by OCA on Thu Feb 21 15:13:57 2008
Categories: Fructose-2,6-bisphosphate 2-phosphatase | Rattus norvegicus | Single protein | Banaszak, L J. | Lange, A J. | Lee, Y H. | Levitt, D G. | Ogata, C M. | Olson, T W. | F6P | Alternative splicing | Atp-binding | Hydrolase | Kinase | Multifunctional enzyme | Multigene family | Phosphorylation | Transferase
