1tjm
From Proteopedia
(New page: 200px<br /><applet load="1tjm" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tjm, resolution 1.18Å" /> '''Crystallographic Ide...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1tjm.jpg|left|200px]]<br /><applet load="1tjm" size=" | + | [[Image:1tjm.jpg|left|200px]]<br /><applet load="1tjm" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1tjm, resolution 1.18Å" /> | caption="1tjm, resolution 1.18Å" /> | ||
'''Crystallographic Identification of Sr2+ Coordination Site in Synaptotagmin I C2B Domain'''<br /> | '''Crystallographic Identification of Sr2+ Coordination Site in Synaptotagmin I C2B Domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Synaptotagmin I has two tandem Ca(2+)-binding C(2) domains, which are | + | Synaptotagmin I has two tandem Ca(2+)-binding C(2) domains, which are essential for fast synchronous synaptic transmission in the central nervous system. We have solved four crystal structures of the C(2)B domain, one of them in the cation-free form at 1.50 A resolution, two in the Ca(2+)-bound form at 1.04 A (two bound Ca(2+) ions) and 1.65 A (three bound Ca(2+) ions) resolution and one in the Sr(2+)-bound form at 1.18 A (one bound Sr(2+) ion) resolution. The side chains of four highly conserved aspartic acids (D303, D309, D363, and D365) and two main chain oxygens (M302:O and Y364:O), together with water molecules, are in direct contact with two bound Ca(2+) ions (sites 1 and 2). At higher Ca(2+) concentrations, the side chain of N333 rotates and cooperates with D309 to generate a third Ca(2+) coordination site (site 3). Divalent cation binding sites 1 and 2 in the C(2)B domain were previously identified from NMR NOE patterns and titration studies, supplemented by site-directed mutation analysis. One difference between the crystal and NMR studies involves D371, which is not involved in coordination with any of the identified Ca(2+) sites in the crystal structures, while it is coordinated to Ca(2+) in site 2 in the NMR structure. In the presence of Sr(2+), which is also capable of triggering exocytosis, but with lower efficiency, only one cation binding site (site 1) was occupied in the crystallographic structure. |
==About this Structure== | ==About this Structure== | ||
| - | 1TJM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with SR and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1TJM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with <scene name='pdbligand=SR:'>SR</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJM OCA]. |
==Reference== | ==Reference== | ||
| Line 15: | Line 15: | ||
[[Category: Cheng, Y.]] | [[Category: Cheng, Y.]] | ||
[[Category: Malinina, L.]] | [[Category: Malinina, L.]] | ||
| - | [[Category: Patel, D | + | [[Category: Patel, D J.]] |
| - | [[Category: Sequeira, S | + | [[Category: Sequeira, S M.]] |
| - | [[Category: Sollner, T | + | [[Category: Sollner, T H.]] |
[[Category: Tereshko, V.]] | [[Category: Tereshko, V.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
| Line 25: | Line 25: | ||
[[Category: synaptotagmin i]] | [[Category: synaptotagmin i]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:14:14 2008'' |
Revision as of 13:14, 21 February 2008
|
Crystallographic Identification of Sr2+ Coordination Site in Synaptotagmin I C2B Domain
Overview
Synaptotagmin I has two tandem Ca(2+)-binding C(2) domains, which are essential for fast synchronous synaptic transmission in the central nervous system. We have solved four crystal structures of the C(2)B domain, one of them in the cation-free form at 1.50 A resolution, two in the Ca(2+)-bound form at 1.04 A (two bound Ca(2+) ions) and 1.65 A (three bound Ca(2+) ions) resolution and one in the Sr(2+)-bound form at 1.18 A (one bound Sr(2+) ion) resolution. The side chains of four highly conserved aspartic acids (D303, D309, D363, and D365) and two main chain oxygens (M302:O and Y364:O), together with water molecules, are in direct contact with two bound Ca(2+) ions (sites 1 and 2). At higher Ca(2+) concentrations, the side chain of N333 rotates and cooperates with D309 to generate a third Ca(2+) coordination site (site 3). Divalent cation binding sites 1 and 2 in the C(2)B domain were previously identified from NMR NOE patterns and titration studies, supplemented by site-directed mutation analysis. One difference between the crystal and NMR studies involves D371, which is not involved in coordination with any of the identified Ca(2+) sites in the crystal structures, while it is coordinated to Ca(2+) in site 2 in the NMR structure. In the presence of Sr(2+), which is also capable of triggering exocytosis, but with lower efficiency, only one cation binding site (site 1) was occupied in the crystallographic structure.
About this Structure
1TJM is a Single protein structure of sequence from Rattus norvegicus with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystallographic identification of Ca2+ and Sr2+ coordination sites in synaptotagmin I C2B domain., Cheng Y, Sequeira SM, Malinina L, Tereshko V, Sollner TH, Patel DJ, Protein Sci. 2004 Oct;13(10):2665-72. Epub 2004 Aug 31. PMID:15340165
Page seeded by OCA on Thu Feb 21 15:14:14 2008
