1tlr
From Proteopedia
(New page: 200px<br /><applet load="1tlr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tlr" /> '''SOLUTION STRUCTURE OF TETRALOOP RECEPTOR RNA...) |
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| - | [[Image:1tlr.jpg|left|200px]]<br /><applet load="1tlr" size=" | + | [[Image:1tlr.jpg|left|200px]]<br /><applet load="1tlr" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1tlr" /> | caption="1tlr" /> | ||
'''SOLUTION STRUCTURE OF TETRALOOP RECEPTOR RNA, NMR, 20 STRUCTURES'''<br /> | '''SOLUTION STRUCTURE OF TETRALOOP RECEPTOR RNA, NMR, 20 STRUCTURES'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The GAAA tetraloop receptor is an 11-nucleotide RNA sequence that | + | The GAAA tetraloop receptor is an 11-nucleotide RNA sequence that participates in the tertiary folding of a variety of large catalytic RNAs by providing a specific binding site for GAAA tetraloops. Here we report the solution structure of the isolated tetraloop receptor as solved by multidimensional, heteronuclear magnetic resonance spectroscopy. The internal loop of the tetraloop receptor has three adenosines stacked in a cross-strand or zipper-like fashion. This arrangement produces a high degree of base stacking within the asymmetric internal loop without extrahelical bases or kinking the helix. Additional interactions within the internal loop include a U. U mismatch pair and a G.U wobble pair. A comparison with the crystal structure of the receptor RNA bound to its tetraloop shows that a conformational change has to occur upon tetraloop binding, which is in good agreement with previous biochemical data. A model for an alternative binding site within the receptor is proposed based on the NMR structure, phylogenetic data and previous crystallographic structures of tetraloop interactions. |
==About this Structure== | ==About this Structure== | ||
| - | 1TLR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1TLR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TLR OCA]. |
==Reference== | ==Reference== | ||
Solution structure of a GAAA tetraloop receptor RNA., Butcher SE, Dieckmann T, Feigon J, EMBO J. 1997 Dec 15;16(24):7490-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9405377 9405377] | Solution structure of a GAAA tetraloop receptor RNA., Butcher SE, Dieckmann T, Feigon J, EMBO J. 1997 Dec 15;16(24):7490-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9405377 9405377] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Butcher, S | + | [[Category: Butcher, S E.]] |
[[Category: Dieckmann, T.]] | [[Category: Dieckmann, T.]] | ||
[[Category: Feigon, J.]] | [[Category: Feigon, J.]] | ||
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[[Category: tetraloop receptor]] | [[Category: tetraloop receptor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:14:54 2008'' |
Revision as of 13:14, 21 February 2008
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SOLUTION STRUCTURE OF TETRALOOP RECEPTOR RNA, NMR, 20 STRUCTURES
Overview
The GAAA tetraloop receptor is an 11-nucleotide RNA sequence that participates in the tertiary folding of a variety of large catalytic RNAs by providing a specific binding site for GAAA tetraloops. Here we report the solution structure of the isolated tetraloop receptor as solved by multidimensional, heteronuclear magnetic resonance spectroscopy. The internal loop of the tetraloop receptor has three adenosines stacked in a cross-strand or zipper-like fashion. This arrangement produces a high degree of base stacking within the asymmetric internal loop without extrahelical bases or kinking the helix. Additional interactions within the internal loop include a U. U mismatch pair and a G.U wobble pair. A comparison with the crystal structure of the receptor RNA bound to its tetraloop shows that a conformational change has to occur upon tetraloop binding, which is in good agreement with previous biochemical data. A model for an alternative binding site within the receptor is proposed based on the NMR structure, phylogenetic data and previous crystallographic structures of tetraloop interactions.
About this Structure
1TLR is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Solution structure of a GAAA tetraloop receptor RNA., Butcher SE, Dieckmann T, Feigon J, EMBO J. 1997 Dec 15;16(24):7490-9. PMID:9405377
Page seeded by OCA on Thu Feb 21 15:14:54 2008
