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1toa
From Proteopedia
(New page: 200px<br /><applet load="1toa" size="450" color="white" frame="true" align="right" spinBox="true" caption="1toa, resolution 1.8Å" /> '''PERIPLASMIC ZINC BIND...) |
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| - | [[Image:1toa.gif|left|200px]]<br /><applet load="1toa" size=" | + | [[Image:1toa.gif|left|200px]]<br /><applet load="1toa" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1toa, resolution 1.8Å" /> | caption="1toa, resolution 1.8Å" /> | ||
'''PERIPLASMIC ZINC BINDING PROTEIN TROA FROM TREPONEMA PALLIDUM'''<br /> | '''PERIPLASMIC ZINC BINDING PROTEIN TROA FROM TREPONEMA PALLIDUM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of recombinant TroA, a zinc-binding protein | + | The crystal structure of recombinant TroA, a zinc-binding protein component of an ATP-binding cassette transport system in Treponema pallidum, was determined at a resolution of 1.8 A. The organization of the protein is largely similar to other periplasmic ligand-binding proteins (PLBP), in that two independent globular domains interact with each other to create a zinc-binding cleft between them. The structure has one bound zinc pentavalently coordinated to residues from both domains. Unlike previous PLBP structures that have an interdomain hinge composed of beta-strands, the N- and C-domains of TroA are linked by a single long backbone helix. This unique backbone helical conformation was possibly adopted to limit the hinge motion associated with ligand exchange. |
==About this Structure== | ==About this Structure== | ||
| - | 1TOA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Treponema_pallidum Treponema pallidum] with ZN and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1TOA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Treponema_pallidum Treponema pallidum] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TOA OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Treponema pallidum]] | [[Category: Treponema pallidum]] | ||
| - | [[Category: Deka, R | + | [[Category: Deka, R K.]] |
| - | [[Category: Hasemann, C | + | [[Category: Hasemann, C A.]] |
| - | [[Category: Lee, Y | + | [[Category: Lee, Y H.]] |
| - | [[Category: Norgard, M | + | [[Category: Norgard, M V.]] |
| - | [[Category: Radolf, J | + | [[Category: Radolf, J D.]] |
[[Category: GOL]] | [[Category: GOL]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
| Line 24: | Line 24: | ||
[[Category: zinc binding protein]] | [[Category: zinc binding protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 15:15:39 2008'' |
Revision as of 13:15, 21 February 2008
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PERIPLASMIC ZINC BINDING PROTEIN TROA FROM TREPONEMA PALLIDUM
Overview
The crystal structure of recombinant TroA, a zinc-binding protein component of an ATP-binding cassette transport system in Treponema pallidum, was determined at a resolution of 1.8 A. The organization of the protein is largely similar to other periplasmic ligand-binding proteins (PLBP), in that two independent globular domains interact with each other to create a zinc-binding cleft between them. The structure has one bound zinc pentavalently coordinated to residues from both domains. Unlike previous PLBP structures that have an interdomain hinge composed of beta-strands, the N- and C-domains of TroA are linked by a single long backbone helix. This unique backbone helical conformation was possibly adopted to limit the hinge motion associated with ligand exchange.
About this Structure
1TOA is a Single protein structure of sequence from Treponema pallidum with and as ligands. Full crystallographic information is available from OCA.
Reference
Treponema pallidum TroA is a periplasmic zinc-binding protein with a helical backbone., Lee YH, Deka RK, Norgard MV, Radolf JD, Hasemann CA, Nat Struct Biol. 1999 Jul;6(7):628-33. PMID:10404217
Page seeded by OCA on Thu Feb 21 15:15:39 2008
